Structure of a Virulence Regulatory Factor CvfB Reveals a Novel Winged Helix RNA Binding Module

Yasuhiko Matsumoto, Qingping Xu, Shinya Miyazaki, Chikara Kaito, Carol L. Farr, Herbert L. Axelrod, Hsiu Ju Chiu, Heath E. Klock, Mark W. Knuth, Mitchell D. Miller, Marc André Elsliger, Ashley M. Deacon, Adam Godzik, Scott A. Lesley, Kazuhisa Sekimizu, Ian A. Wilson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

CvfB is a conserved regulatory protein important for the virulence of Staphylococcus aureus. We show here that CvfB binds RNA. The crystal structure of the CvfB ortholog from Streptococcus pneumoniae at 1.4 Å resolution reveals a unique RNA binding protein that is formed from a concatenation of well-known structural modules that bind nucleic acids: three consecutive S1 RNA binding domains and a winged helix (WH) domain. The third S1 and the WH domains are required for cooperative RNA binding and form a continuous surface that likely contributes to the RNA interaction. The WH domain is critical to CvfB function and contains a unique sequence motif. Thus CvfB represents a novel assembly of modules for binding RNA.

Original languageEnglish
Pages (from-to)537-547
Number of pages11
JournalStructure
Volume18
Issue number4
DOIs
Publication statusPublished - Mar 1 2010
Externally publishedYes

Keywords

  • Microbio
  • Proteins
  • RNA

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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  • Cite this

    Matsumoto, Y., Xu, Q., Miyazaki, S., Kaito, C., Farr, C. L., Axelrod, H. L., Chiu, H. J., Klock, H. E., Knuth, M. W., Miller, M. D., Elsliger, M. A., Deacon, A. M., Godzik, A., Lesley, S. A., Sekimizu, K., & Wilson, I. A. (2010). Structure of a Virulence Regulatory Factor CvfB Reveals a Novel Winged Helix RNA Binding Module. Structure, 18(4), 537-547. https://doi.org/10.1016/j.str.2010.02.007