TY - JOUR
T1 - Structure of a tetrameric photosystem I from a glaucophyte alga Cyanophora paradoxa
AU - Kato, Koji
AU - Nagao, Ryo
AU - Ueno, Yoshifumi
AU - Yokono, Makio
AU - Suzuki, Takehiro
AU - Jiang, Tian Yi
AU - Dohmae, Naoshi
AU - Akita, Fusamichi
AU - Akimoto, Seiji
AU - Miyazaki, Naoyuki
AU - Shen, Jian Ren
N1 - Funding Information:
We thank Drs. Naruhiko Adachi and Masato Kawasaki for helpful assistance during the cryo-EM study. This work was supported by the Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED, JSPS KAKENHI grant Nos. JP20H02914 (K.K.), JP20K06528, JP21K19085 (R.N.), JP18J10095 (Y.U.), JP19K22396, JP20H03194 (F.A.), JP16H06553 (S.A.), JP20H05087 (N.M.), and JP17H06433 (J.-R.S.), Takeda Science Foundation (K.K.), and TIA-Kakehashi grant No. TK19-048 (N.M.).
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Photosystem I (PSI) is one of the two photosystems functioning in light-energy harvesting, transfer, and electron transfer in photosynthesis. However, the oligomerization state of PSI is variable among photosynthetic organisms. We present a 3.8-Å resolution cryo-electron microscopic structure of tetrameric PSI isolated from the glaucophyte alga Cyanophora paradoxa, which reveals differences with PSI from other organisms in subunit composition and organization. The PSI tetramer is organized in a dimer of dimers with a C2 symmetry. Unlike cyanobacterial PSI tetramers, two of the four monomers are rotated around 90°, resulting in a completely different pattern of monomer-monomer interactions. Excitation-energy transfer among chlorophylls differs significantly between Cyanophora and cyanobacterial PSI tetramers. These structural and spectroscopic features reveal characteristic interactions and excitation-energy transfer in the Cyanophora PSI tetramer, suggesting that the Cyanophora PSI could represent a turning point in the evolution of PSI from prokaryotes to eukaryotes.
AB - Photosystem I (PSI) is one of the two photosystems functioning in light-energy harvesting, transfer, and electron transfer in photosynthesis. However, the oligomerization state of PSI is variable among photosynthetic organisms. We present a 3.8-Å resolution cryo-electron microscopic structure of tetrameric PSI isolated from the glaucophyte alga Cyanophora paradoxa, which reveals differences with PSI from other organisms in subunit composition and organization. The PSI tetramer is organized in a dimer of dimers with a C2 symmetry. Unlike cyanobacterial PSI tetramers, two of the four monomers are rotated around 90°, resulting in a completely different pattern of monomer-monomer interactions. Excitation-energy transfer among chlorophylls differs significantly between Cyanophora and cyanobacterial PSI tetramers. These structural and spectroscopic features reveal characteristic interactions and excitation-energy transfer in the Cyanophora PSI tetramer, suggesting that the Cyanophora PSI could represent a turning point in the evolution of PSI from prokaryotes to eukaryotes.
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U2 - 10.1038/s41467-022-29303-7
DO - 10.1038/s41467-022-29303-7
M3 - Article
C2 - 35354806
AN - SCOPUS:85127231329
VL - 13
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 1679
ER -