Structure of a green algal photosystem I in complex with a large number of light-harvesting complex I subunits

Xiaochun Qin, Xiong Pi, Wenda Wang, Guangye Han, Lixia Zhu, Mingmei Liu, Linpeng Cheng, Jian-Ren Shen, Tingyun Kuang, Sen Fang Sui

Research output: Contribution to journalArticle

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Abstract

Photosystem I (PSI) is a highly efficient natural light-energy converter, and has diverse light-harvesting antennas associated with its core in different photosynthetic organisms. In green algae, an extremely large light-harvesting complex I (LHCI) captures and transfers energy to the PSI core. Here, we report the structure of PSI–LHCI from a green alga Bryopsis corticulans at 3.49 Å resolution, obtained by single-particle cryo-electron microscopy, which revealed 13 core subunits including subunits characteristic of both prokaryotes and eukaryotes, and 10 light-harvesting complex a (Lhca) antennas that form a double semi-ring and an additional Lhca dimer, including a novel four-transmembrane-helix Lhca. In total, 244 chlorophylls were identified, some of which were located at key positions for the fast energy transfer. These results provide a firm structural basis for unravelling the mechanisms of light-energy harvesting, transfer and quenching in the green algal PSI–LHCI, and important clues as to how PSI–LHCI has changed during evolution.

Original languageEnglish
Pages (from-to)263-272
Number of pages10
JournalNature Plants
Volume5
Issue number3
DOIs
Publication statusPublished - Mar 1 2019

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light harvesting complex
photosystem I
energy transfer
Chlorophyta
Bryopsis
energy conversion
autotrophs
prokaryotic cells
eukaryotic cells
solar radiation
chlorophyll
energy

ASJC Scopus subject areas

  • Plant Science

Cite this

Structure of a green algal photosystem I in complex with a large number of light-harvesting complex I subunits. / Qin, Xiaochun; Pi, Xiong; Wang, Wenda; Han, Guangye; Zhu, Lixia; Liu, Mingmei; Cheng, Linpeng; Shen, Jian-Ren; Kuang, Tingyun; Sui, Sen Fang.

In: Nature Plants, Vol. 5, No. 3, 01.03.2019, p. 263-272.

Research output: Contribution to journalArticle

Qin, X, Pi, X, Wang, W, Han, G, Zhu, L, Liu, M, Cheng, L, Shen, J-R, Kuang, T & Sui, SF 2019, 'Structure of a green algal photosystem I in complex with a large number of light-harvesting complex I subunits', Nature Plants, vol. 5, no. 3, pp. 263-272. https://doi.org/10.1038/s41477-019-0379-y
Qin, Xiaochun ; Pi, Xiong ; Wang, Wenda ; Han, Guangye ; Zhu, Lixia ; Liu, Mingmei ; Cheng, Linpeng ; Shen, Jian-Ren ; Kuang, Tingyun ; Sui, Sen Fang. / Structure of a green algal photosystem I in complex with a large number of light-harvesting complex I subunits. In: Nature Plants. 2019 ; Vol. 5, No. 3. pp. 263-272.
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