Structure and molecular characterization of barley nudix hydrolase genes

Sayuri Tanaka, Makoto Kihara, Manabu Sugimoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Putative nudix hydrolase (NUDX) genes, which encode amino acid sequences showing homology with those of Arabidopsis NUDXs and conserve nudix motif, were identified from barley. The 14 deduced barley NUDXs (HvNUDX1-14) were classified into established subfamilies, except for 8-oxo-deoxyguanosine 5′-triphosphate (8-oxo-dGTP) pyrophosphohydrolase and mRNA decapping enzyme subfamilies, and three substrate-unknown subfamilies. Drought and UV-C stresses, respectively, up-regulated 7 and 4 HvNUDX genes, but some homologs of Arabidopsis NUDXs showed different responses to abiotic stress. HvNUDX12 gene, belonging to diadenosine tetraphosphates (Ap4A) pyrophosphohydrolase subfamily gene and up-regulated by UV-C, was expressed in Escherichia coli cells. The recombinant protein showed 8-oxo-dGTP, Ap4A, and guanosine-3′,5′-tetraphosphate (ppGpp) pyrophosphohydrolase activities, and the suppression of the lacZ amber mutation in a mutT-deficient E. coli cells caused by the incorporation of 8-oxo-GTP into mRNA was prevented to a significant degree. These results suggest that barley NUDXs have unique constitution and response of NUDX to abiotic stress.

Original languageEnglish
Pages (from-to)394-401
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Hordeum
Molecular Structure
Genes
Arabidopsis
Escherichia coli
Amino Acid Sequence Homology
Guanosine
Drought
Droughts
Constitution and Bylaws
Guanosine Triphosphate
Recombinant Proteins
Amino Acids
Messenger RNA
Mutation
nudix hydrolases
Substrates
diadenosine tetraphosphate
deoxyguanosine triphosphate
5'-deoxyguanosine

Keywords

  • 8-oxo-dGTP
  • Abiotic stress
  • Barley
  • Nudix hydrolase
  • UV-C

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry
  • Medicine(all)

Cite this

Structure and molecular characterization of barley nudix hydrolase genes. / Tanaka, Sayuri; Kihara, Makoto; Sugimoto, Manabu.

In: Bioscience, Biotechnology and Biochemistry, Vol. 79, No. 3, 2015, p. 394-401.

Research output: Contribution to journalArticle

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