Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus

Shigeo Yamamoto, Noriyuki Okujo, Takashi Yoshida, Shiro Matsuura, Sumio Shinoda

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The structure of vibrioferrin, a siderophore from Vibrio parahaemolyticus, was elucidated based on a combination of partial hydrolysis and spectroscopic techniques. HPLC of purified vibrioferrin showed two peaks with an area ratio of approximately 2:1. However, upon reinjection of each of those isolated compounds, the original chromatographic pattern was obtained, indicating an equilibrium between two compounds in aqueous solution. Consistent with this finding, most of the NMR signals of vibrioferrin were duplicated. The structure was determined as 1-{2-[2-(5-carboxy-5-hydroxy-2-oxo-l-pyrrolidinyl)pro-pionamide]ethyl} citrate, which exists in two epimeric forms resulting from cyclization between an amidic nitrogen of the alanine residue and a keto group of the 2-ketoglutaric acid residue. Transport experiments with 56Fe-labeled vibrioferrin demonstrated the function of vibrioferrin as a siderophore in V. parahaemolyticus. Kinetic studies with mid-log phase cells revealed that the iron uptake system was receptor-mediated, with Km and Vmax values of 67 nM and 54 pmol Fe/mg cell protein/min, respectively. Moreover, iron uptake mediated by vibrioferrin was blocked both by uncouplers and by ATPase inhibitors.

Original languageEnglish
Pages (from-to)868-874
Number of pages7
JournalJournal of Biochemistry
Volume115
Issue number5
Publication statusPublished - May 1994

Fingerprint

Vibrio parahaemolyticus
Siderophores
Iron
High-performance Liquid Chromatography
ATP Synthase
Cyclization
Cell
Receptor
Nitrogen
Inhibitor
Hydrolysis
Kinetics
Nuclear magnetic resonance
Proteins
Protein
Partial
Acids
Experiment
Experiments
Alanine

Keywords

  • Iron uptake
  • Siderophore
  • Vibrio parahaemolyticus
  • Vibrioferrin

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Yamamoto, S., Okujo, N., Yoshida, T., Matsuura, S., & Shinoda, S. (1994). Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. Journal of Biochemistry, 115(5), 868-874.

Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. / Yamamoto, Shigeo; Okujo, Noriyuki; Yoshida, Takashi; Matsuura, Shiro; Shinoda, Sumio.

In: Journal of Biochemistry, Vol. 115, No. 5, 05.1994, p. 868-874.

Research output: Contribution to journalArticle

Yamamoto, S, Okujo, N, Yoshida, T, Matsuura, S & Shinoda, S 1994, 'Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus', Journal of Biochemistry, vol. 115, no. 5, pp. 868-874.
Yamamoto S, Okujo N, Yoshida T, Matsuura S, Shinoda S. Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. Journal of Biochemistry. 1994 May;115(5):868-874.
Yamamoto, Shigeo ; Okujo, Noriyuki ; Yoshida, Takashi ; Matsuura, Shiro ; Shinoda, Sumio. / Structure and iron transport activity of vibrioferrin, a new siderophore of Vibrio parahaemolyticus. In: Journal of Biochemistry. 1994 ; Vol. 115, No. 5. pp. 868-874.
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