Structure and conservation of a polyethylene glycol-degradative operon in sphingomonads

Akiwo Tani, Jittima Charoenpanich, Terumi Mori, Mayuko Takeichi, Kazuhide Kimbara, Fusako Kawai

Research output: Contribution to journalArticle

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Abstract

Sphingopyxis terrae, and Sphingopyxis macrogoltabida strains 103 and 203, can degrade polyethylene glycols (PEGs). They differ in the following respects: (i) different substrate specificities (chain length) of assimilable PEG, (ii) PEG-inducible or constitutive PEG-degradative proteins, and (iii) symbiotic or axenic degradation of PEG. S. terrae was able to incorporate PEG 6000, but strain 103 could not incorporate more than PEG 4000, suggesting that the difference in assimilable PEG chain length depends on the ability to take up substrate. PEG-degradative genes (pegB, C, D, A, E and R) from these strains were cloned. Their primary structures shared a high homology of more than 99%. The peg genes encode a TonB-dependent receptor (pegB), a PEG-aldehyde dehydrogenase (pegC), a permease (pegD), a PEG dehydrogenase (pegA) and an acyl-CoA ligase (pegE), and in the opposite orientation, an AraC-type transcription regulator (pegR). The peg operon was flanked by two different sets of transposases. These three strains contained large plasmids and the operon was located in one of the large plasmids in S. terrae. The peg genes could be detected in other PEG-degrading sphingomonads. These results suggest that the peg genes have evolved in a plasmid-mediated manner. An insertion of a transposon gene (pegF) between pegD and pegA in strain 203 was found, which caused the constitutive expression of pegA in this strain).

Original languageEnglish
Pages (from-to)338-346
Number of pages9
JournalMicrobiology
Volume153
Issue number2
DOIs
Publication statusPublished - Feb 2007

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Operon
Plasmids
Genes
Glycolaldehyde Dehydrogenase
AraC Transcription Factor
Transposases
Acyl Coenzyme A
Membrane Transport Proteins
Ligases
Substrate Specificity

ASJC Scopus subject areas

  • Microbiology

Cite this

Structure and conservation of a polyethylene glycol-degradative operon in sphingomonads. / Tani, Akiwo; Charoenpanich, Jittima; Mori, Terumi; Takeichi, Mayuko; Kimbara, Kazuhide; Kawai, Fusako.

In: Microbiology, Vol. 153, No. 2, 02.2007, p. 338-346.

Research output: Contribution to journalArticle

Tani, A, Charoenpanich, J, Mori, T, Takeichi, M, Kimbara, K & Kawai, F 2007, 'Structure and conservation of a polyethylene glycol-degradative operon in sphingomonads', Microbiology, vol. 153, no. 2, pp. 338-346. https://doi.org/10.1099/mic.0.2006/000992-0
Tani, Akiwo ; Charoenpanich, Jittima ; Mori, Terumi ; Takeichi, Mayuko ; Kimbara, Kazuhide ; Kawai, Fusako. / Structure and conservation of a polyethylene glycol-degradative operon in sphingomonads. In: Microbiology. 2007 ; Vol. 153, No. 2. pp. 338-346.
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