Structure and assembly of double-stranded RNA mycoviruses

Carlos P. Mata, Javier M. Rodríguez, Nobuhiro Suzuki, José R. Castón

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Mycoviruses are a diverse group that includes ssRNA, dsRNA, and ssDNA viruses, with or without a protein capsid, as well as with a complex envelope. Most mycoviruses are transmitted by cytoplasmic interchange and are thought to lack an extracellular phase in their infection cycle. Structural analysis has focused on dsRNA mycoviruses, which usually package their genome in a 120-subunit T = 1 icosahedral capsid, with a capsid protein (CP) dimer as the asymmetric unit. The atomic structure is available for four dsRNA mycovirus from different families: Saccharomyces cerevisiae virus L-A (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). Their capsids show structural variations of the same framework, with asymmetric or symmetric CP dimers respectively for ScV-L-A and PsV-F, dimers of similar domains of a single CP for PcV, or of two different proteins for RnQV1. The CP dimer is the building block, and assembly proceeds through dimers of dimers or pentamers of dimers, in which the genome is packed as ssRNA by interaction with CP and/or viral polymerase. These capsids remain structurally undisturbed throughout the viral cycle. The T = 1 capsid participates in RNA synthesis, organizing the viral polymerase (1–2 copies) and a single loosely packaged genome segment. It also acts as a molecular sieve, to allow the passage of viral transcripts and nucleotides, but to prevent triggering of host defense mechanisms. Due to the close mycovirus-host relationship, CP evolved to allocate peptide insertions with enzyme activity, as reflected in a rough outer capsid surface.

    Original languageEnglish
    Title of host publicationAdvances in Virus Research
    EditorsMargaret Kielian, Thomas C. Mettenleiter, Marilyn J. Roossinck
    PublisherAcademic Press Inc.
    Pages213-247
    Number of pages35
    ISBN (Print)9780128207611
    DOIs
    Publication statusPublished - Jan 2020

    Publication series

    NameAdvances in Virus Research
    Volume108
    ISSN (Print)0065-3527
    ISSN (Electronic)1557-8399

    Keywords

    • Capsid assembly
    • Capsid protein
    • PcV
    • PsV-F
    • RnQV1
    • ScV-L-A
    • Virus evolution
    • dsRNA mycovirus

    ASJC Scopus subject areas

    • Virology
    • Infectious Diseases

    Fingerprint

    Dive into the research topics of 'Structure and assembly of double-stranded RNA mycoviruses'. Together they form a unique fingerprint.

    Cite this