Structural features of N-glycans of seaweed glycoproteins: Predominant occurrence of high-mannose type N-glycans in marine plants

Takeo Yoshiie, Megumi Maeda, Mariko Kimura, Yoichiro Hama, Motoharu Uchida, Yoshinobu Kimura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We analyzed structural features of N-glycans linked to glycoproteins expressed in various seaweeds to identify new sources of biologically-important N-glycans or N-glycopeptides. Structural analysis of the N-glycans of glycopeptides prepared from pepsin digests of 15 species of seaweed revealed that only high-mannose type N-glycans occur in seaweed glycoproteins, and the Man9GlcNAc2 structure predominates in Sargassum fulvellum and Zostera marina, while no typical plant complex type N-glycans bearing β1-2 xylosyl and α1-3 fucosyl residues present in either algae or seagrass. These results indicate that seaweeds lack the activities of several of the glycosyltransferases required for the biosynthesis of the complex type N-glycans found in terrestrial plants, and that the context of N-glycan processing in seaweeds is different from that in terrestrial plant cells.

Original languageEnglish
Pages (from-to)1996-1998
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number10
DOIs
Publication statusPublished - 2012

Keywords

  • High-mannose type N-glycan
  • Marine algae
  • Marine plants
  • Seaweed N-glycan
  • Seaweed glycoprotein

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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