Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen

Yoshinobu Kimura, Takeo Yoshiie, Woo Kwan Kit, Megumi Maeda, Mariko Kimura, Siang Hee Tan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The pollen of oil palm (Elaeis guineensis Jacq.) is a strong allergen and causes severe pollinosis in Malaysia and Singapore. In the previous study (Biosci. Biotechnol. Biochem., 64, 820-827 (2002)), from the oil palm pollens, we purified an antigenic glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients. In this report, we describe the structural analysis of sugar chains linked to palm pollen glycoproteins to confirm the ubiquitous occurrence of antigenic N-glycans in the allergenic pollen. N-Glycans liberated from the pollen glycoprotein mixture by hydrazinolysis were labeled with 2-aminopyridine followed by purification with a combination of size-fractionation HPLC and reversed-phase HPLC. The structures of the PA-sugar chains were analyzed by a combination of two-dimensional sugar chain mapping, electrospray ionization mass spectrometry (ESI-MS), and tandem MS analysis, as well as exoglycosidase digestions. The antigenic N-glycan bearing α1-3 fucose and/or β1-2 xylose residues accounts for 36.9% of total N-glycans: GlcNAc2Man3Xyl1Fuc1GlcNAc 2 (24.6%), GlcNAc2Man3Xyl 1GlcNAc2 (4.4%), Man3Xyl1Fuc 1GlcNAc2 (1.1%), GlcNAc1Man3Xyl 1Fuc1GlcNAc2 (5.6%), and GlcNAc 1Man3Xyl1GlcNAc2 (1.2%). The remaining 63.1% of the total N-glycans belong to the high-mannose type structure: Man9GlcNAc2 (5.8%), Man8GlcNAc2 (32.1%), Man7GlcNAc2 (19.9%), Man6GlcNAc2 (5.3%).

Original languageEnglish
Pages (from-to)2232-2239
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number10
DOIs
Publication statusPublished - Oct 2003

Fingerprint

Glycoproteins
Palm oil
Elaeis guineensis
Pollen
Sugars
Polysaccharides
glycoproteins
Oils
polysaccharides
pollen
hay fever
Bearings (structural)
Seasonal Allergic Rhinitis
Allergens
Electrospray ionization
sugars
Xylose
Fractionation
Structural analysis
Purification

Keywords

  • Antigenic N-glycan
  • Elaeis guineensis Jacq
  • Oil palm pollen
  • Plant glycoprotein
  • Pollen allergy

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen. / Kimura, Yoshinobu; Yoshiie, Takeo; Kit, Woo Kwan; Maeda, Megumi; Kimura, Mariko; Tan, Siang Hee.

In: Bioscience, Biotechnology and Biochemistry, Vol. 67, No. 10, 10.2003, p. 2232-2239.

Research output: Contribution to journalArticle

Kimura, Yoshinobu ; Yoshiie, Takeo ; Kit, Woo Kwan ; Maeda, Megumi ; Kimura, Mariko ; Tan, Siang Hee. / Structural features of N-glycans linked to glycoproteins from oil palm pollen, an allergenic pollen. In: Bioscience, Biotechnology and Biochemistry. 2003 ; Vol. 67, No. 10. pp. 2232-2239.
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