Structural features of free N-glycans in α1,3/4-fucosidase-deficient Arabidopsis thaliana: deletion of α1,3/4-fucosidase activity induced accumulation of plant complex type GN1 free N-glycans

Shun Takata, Megumi Hayashi, Megumi Maeda, Takeshi Ishimizu, Yoshinobu Kimura

Research output: Contribution to journalArticlepeer-review

Abstract

Deletion of α-1,3/4-fucosidase activity in Arabidopsis thaliana resulted in the accumulation of GN1-type free N-glycans with the Lewis a epitope (GN1-FNG). This suggests that the release of α-fucose residue(s) may trigger rapid degradation of the plant complex-type (PCT) GN1-FNG. The fact that PCT-GN1-FNG has rarely been detected to date is probably due to its easier degradation compared with PCT-GN2-FNG.

Original languageEnglish
Pages (from-to)1413-1416
Number of pages4
JournalBioscience, biotechnology, and biochemistry
Volume86
Issue number10
DOIs
Publication statusPublished - Sep 23 2022

Keywords

  • Arabidopsis thaliana
  • endo-β-N-acetylglucosaminidase
  • free N-glycans
  • peptide: N-glycanase
  • α-fucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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