Structural evidence of a productive active site architecture for an evolved quorum-quenching GKL lactonase

Bo Xue, Jeng Yeong Chow, Amgalanbaatar Baldansuren, Lai Lai Yap, Yunn Hwen Gan, Sergei A. Dikanov, Robert C. Robinson, Wen Shan Yew

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The in vitro evolution and engineering of quorum-quenching lactonases with enhanced reactivities was achieved using a thermostable GKL enzyme as a template, yielding the E101G/R230C GKL mutant with increased catalytic activity and a broadened substrate range [Chow, J. Y., Xue, B., Lee, K. H., Tung, A., Wu, L., Robinson, R. C., and Yew, W. S. (2010) J. Biol. Chem. 285, 40911-40920]. This enzyme possesses the (β/α)8-barrel fold and is a member of the PLL (phosphotriesterase-like lactonase) group of enzymes within the amidohydrolase superfamily that hydrolyze N-acyl-homoserine lactones, which mediate the quorum-sensing pathways of bacteria. The structure of the evolved N-butyryl-l-homoserine lactone (substrate)-bound E101G/R230C GKL enzyme was determined, in the presence of the inactivating D266N mutation, to a resolution of 2.2 Å to provide an explanation for the observed rate enhancements. In addition, the substrate-bound structure of the catalytically inactive E101N/D266N mutant of the manganese-reconstituted enzyme was determinied to a resolution of 2.1 Å and the structure of the ligand-free, manganese-reconstituted E101N mutant to a resolution of 2.6 Å, and the structures of ligand-free zinc-reconstituted wild-type, E101N, R230D, and E101G/R230C mutants of GKL were determinied to resolutions of 2.1, 2.1, 1.9, and 2.0 Å, respectively. In particular, the structure of the evolved E101G/R230C mutant of GKL provides evidence of a catalytically productive active site architecture that contributes to the observed enhancement of catalysis. At high concentrations, wild-type and mutant GKL enzymes are differentially colored, with absorbance maxima in the range of 512-553 nm. The structures of the wild-type and mutant GKL provide a tractable link between the origins of the coloration and the charge-transfer complex between the α-cation and Tyr99 within the enzyme active site. Taken together, this study provides evidence of the modulability of enzymatic catalysis through subtle changes in enzyme active site architecture.

Original languageEnglish
Pages (from-to)2359-2370
Number of pages12
JournalBiochemistry
Volume52
Issue number13
DOIs
Publication statusPublished - Apr 2 2013
Externally publishedYes

Fingerprint

Quorum Sensing
Quenching
Catalytic Domain
Enzymes
Manganese
Catalysis
Phosphoric Triester Hydrolases
Substrates
Acyl-Butyrolactones
Amidohydrolases
Ligands
Cations
Charge transfer
Zinc
Catalyst activity
Bacteria
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Xue, B., Chow, J. Y., Baldansuren, A., Yap, L. L., Gan, Y. H., Dikanov, S. A., ... Yew, W. S. (2013). Structural evidence of a productive active site architecture for an evolved quorum-quenching GKL lactonase. Biochemistry, 52(13), 2359-2370. https://doi.org/10.1021/bi4000904

Structural evidence of a productive active site architecture for an evolved quorum-quenching GKL lactonase. / Xue, Bo; Chow, Jeng Yeong; Baldansuren, Amgalanbaatar; Yap, Lai Lai; Gan, Yunn Hwen; Dikanov, Sergei A.; Robinson, Robert C.; Yew, Wen Shan.

In: Biochemistry, Vol. 52, No. 13, 02.04.2013, p. 2359-2370.

Research output: Contribution to journalArticle

Xue, B, Chow, JY, Baldansuren, A, Yap, LL, Gan, YH, Dikanov, SA, Robinson, RC & Yew, WS 2013, 'Structural evidence of a productive active site architecture for an evolved quorum-quenching GKL lactonase', Biochemistry, vol. 52, no. 13, pp. 2359-2370. https://doi.org/10.1021/bi4000904
Xue, Bo ; Chow, Jeng Yeong ; Baldansuren, Amgalanbaatar ; Yap, Lai Lai ; Gan, Yunn Hwen ; Dikanov, Sergei A. ; Robinson, Robert C. ; Yew, Wen Shan. / Structural evidence of a productive active site architecture for an evolved quorum-quenching GKL lactonase. In: Biochemistry. 2013 ; Vol. 52, No. 13. pp. 2359-2370.
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AU - Gan, Yunn Hwen

AU - Dikanov, Sergei A.

AU - Robinson, Robert C.

AU - Yew, Wen Shan

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