Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome

Yasunari Ogihara, Yukiko Yamazaki, Koji Murai, Akira Kanno, Toru Terachi, Takashi Shiina, Naohiko Miyashita, Shuhei Nasuda, Chiharu Nakamura, Naoki Mori, Shigeo Takumi, Minoru Murata, Satoshi Futo, Koichiro Tsunewaki

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

The application of a new gene-based strategy for sequencing the wheat mitochondrial genome shows its structure to be a 452 528 bp circular molecule, and provides nucleotide-level evidence of intra-molecular recombination. Single, reciprocal and double recombinant products, and the nucleotide sequences of the repeats that mediate their formation have been identified. The genome has 55 genes with exons, including 35 protein-coding, 3 rRNA and 17 tRNA genes. Nucleotide sequences of seven wheat genes have been determined here for the first time. Nine genes have an exon-intron structure. Gene amplification responsible for the production of multicopy mitochondrial genes, in general, is species-specific, suggesting the recent origin of these genes. About 16, 17, 15, 3.0 and 0.2% of wheat mitochondrial DNA (mtDNA) may be of genic (including introns), open reading frame, repetitive sequence, chloroplast and retro-element origin, respectively. The gene order of the wheat mitochondrial gene map shows little synteny to the rice and maize maps, indicative that thorough gene shuffling occurred during speciation. Almost all unique mtDNA sequences of wheat, as compared with rice and maize mtDNAs, are redundant DNA. Features of the gene-based strategy are discussed, and a mechanistic model of mitochondrial gene amplification is proposed.

Original languageEnglish
Pages (from-to)6235-6250
Number of pages16
JournalNucleic acids research.
Volume33
Issue number19
DOIs
Publication statusPublished - 2005

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Mitochondrial Genome
Triticum
Nucleotides
Genes
Mitochondrial Genes
Gene Amplification
Mitochondrial DNA
Introns
Zea mays
Exons
Synteny
Gene Order
Edible Grain
Nucleic Acid Repetitive Sequences
Chloroplasts
Transfer RNA
Genetic Recombination
Open Reading Frames
Genome
DNA

ASJC Scopus subject areas

  • Genetics

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Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome. / Ogihara, Yasunari; Yamazaki, Yukiko; Murai, Koji; Kanno, Akira; Terachi, Toru; Shiina, Takashi; Miyashita, Naohiko; Nasuda, Shuhei; Nakamura, Chiharu; Mori, Naoki; Takumi, Shigeo; Murata, Minoru; Futo, Satoshi; Tsunewaki, Koichiro.

In: Nucleic acids research., Vol. 33, No. 19, 2005, p. 6235-6250.

Research output: Contribution to journalArticle

Ogihara, Y, Yamazaki, Y, Murai, K, Kanno, A, Terachi, T, Shiina, T, Miyashita, N, Nasuda, S, Nakamura, C, Mori, N, Takumi, S, Murata, M, Futo, S & Tsunewaki, K 2005, 'Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome', Nucleic acids research., vol. 33, no. 19, pp. 6235-6250. https://doi.org/10.1093/nar/gki925
Ogihara, Yasunari ; Yamazaki, Yukiko ; Murai, Koji ; Kanno, Akira ; Terachi, Toru ; Shiina, Takashi ; Miyashita, Naohiko ; Nasuda, Shuhei ; Nakamura, Chiharu ; Mori, Naoki ; Takumi, Shigeo ; Murata, Minoru ; Futo, Satoshi ; Tsunewaki, Koichiro. / Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome. In: Nucleic acids research. 2005 ; Vol. 33, No. 19. pp. 6235-6250.
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