Structural characterization of neutral and anionic glucans from Mesorhizobium loti

Yasuyuki Kawaharada, Hiromasa Kiyota, Shima Eda, Kiwamu Minamisawa, Hisayuki Mitsui

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The periplasmic glucans of Mesorhizobium loti were isolated and separated into fractions according to their acidity. NMR spectroscopy confirmed their backbone structure to be a cyclic β-(1→2)-d-glucan as in the case of other rhizobia, and revealed no non-glycosidic substituents in the neutral fraction, and glycerophosphoryl and succinyl residues as major and minor substituents, respectively, in the anionic fractions. MALDI-TOF mass spectrometry showed that the anionic glucans contain one, two, or three such substituents per molecule according to their acidity, and, in contrast, that all the anionic subfractions have a similar size distribution to that of the neutral glucans, where molecules composed of 20-24 glucosyl residues are predominant. These results clarify the periplasmic glucan composition in terms of charge-to-mass ratios in M. loti cells.

Original languageEnglish
Pages (from-to)2422-2427
Number of pages6
JournalCarbohydrate Research
Volume343
Issue number14
DOIs
Publication statusPublished - Sep 22 2008
Externally publishedYes

Keywords

  • Cyclic glucan
  • Glycerophosphoryl substituent
  • Mesorhizobium loti
  • Succinyl substituent
  • β-(1→2)-Glycosidic linkage

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Structural characterization of neutral and anionic glucans from Mesorhizobium loti'. Together they form a unique fingerprint.

  • Cite this