Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin

Tomoyuki Konishi, Fumiko Taguchi, Masako Iwaki, Mayumi Ohnishi-Kameyama, Masanobu Yamamoto, Ikuko Maeda, Yoshihiro Nishida, Yuki Ichinose, Mitsuru Yoshida, Tadashi Ishii

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The flagellin of Pseudomonas syringae pv. tabaci is a glycoprotein that contains O-linked oligosaccharides composed of rhamnosyl and 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methylglucosyl residues. These O-linked glycans are released by hydrazinolysis and then labeled at their reducing ends with 2-aminopyridine (PA). A PA-labeled trisaccharide and a PA-labeled tetrasaccharide are isolated by normal-phase high-performance liquid chromatography. These oligosaccharides are structurally characterized using mass spectrometry and NMR spectroscopy. Our data show that P. syringae pv. tabaci flagellin is glycosylated with a tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-l-Rhap-(1→2)-α-l-Rhap-(1→2)-α-l-Rha-(1→, as well a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-l-Rhap-(1→2)-α-l-Rha-(1→, which was identified in a previous study.

Original languageEnglish
Pages (from-to)2250-2254
Number of pages5
JournalCarbohydrate Research
Volume344
Issue number16
DOIs
Publication statusPublished - Nov 2 2009

Fingerprint

Pseudomonas syringae
Flagellin
Trisaccharides
Oligosaccharides
Mass Spectrometry
High performance liquid chromatography
Nuclear magnetic resonance spectroscopy
Mass spectrometry
Polysaccharides
Glycoproteins
Magnetic Resonance Spectroscopy
High Pressure Liquid Chromatography
alpha-aminopyridine

Keywords

  • Bacteria
  • Flagellin glycosylation
  • NMR
  • O-linked oligosaccharides
  • Pseudomonas syringae
  • Structure

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Konishi, T., Taguchi, F., Iwaki, M., Ohnishi-Kameyama, M., Yamamoto, M., Maeda, I., ... Ishii, T. (2009). Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin. Carbohydrate Research, 344(16), 2250-2254. https://doi.org/10.1016/j.carres.2009.07.004

Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin. / Konishi, Tomoyuki; Taguchi, Fumiko; Iwaki, Masako; Ohnishi-Kameyama, Mayumi; Yamamoto, Masanobu; Maeda, Ikuko; Nishida, Yoshihiro; Ichinose, Yuki; Yoshida, Mitsuru; Ishii, Tadashi.

In: Carbohydrate Research, Vol. 344, No. 16, 02.11.2009, p. 2250-2254.

Research output: Contribution to journalArticle

Konishi, T, Taguchi, F, Iwaki, M, Ohnishi-Kameyama, M, Yamamoto, M, Maeda, I, Nishida, Y, Ichinose, Y, Yoshida, M & Ishii, T 2009, 'Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin', Carbohydrate Research, vol. 344, no. 16, pp. 2250-2254. https://doi.org/10.1016/j.carres.2009.07.004
Konishi, Tomoyuki ; Taguchi, Fumiko ; Iwaki, Masako ; Ohnishi-Kameyama, Mayumi ; Yamamoto, Masanobu ; Maeda, Ikuko ; Nishida, Yoshihiro ; Ichinose, Yuki ; Yoshida, Mitsuru ; Ishii, Tadashi. / Structural characterization of an O-linked tetrasaccharide from Pseudomonas syringae pv. tabaci flagellin. In: Carbohydrate Research. 2009 ; Vol. 344, No. 16. pp. 2250-2254.
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AU - Yamamoto, Masanobu

AU - Maeda, Ikuko

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