Structural characterization of a capping protein interaction motif defines a family of actin filament regulators

Maria Hernandez-Valladares, Taekyung Kim, Balakrishnan Kannan, Alvin Tung, Adeleke H. Aguda, Mrten Larsson, John A. Cooper, Robert C. Robinson

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Capping protein (CP) regulates actin dynamics by binding the barbed ends of actin filaments. Removal of CP may be one means to harness actin polymerization for processes such as cell movement and endocytosis. Here we structurally and biochemically investigated a CP interaction (CPI) motif present in the otherwise unrelated proteins CARMIL and CD2AP. The CPI motif wraps around the stalk of the mushroom-shaped CP at a site distant from the actin-binding interface, which lies on the top of the mushroom cap. We propose that the CPI motif may act as an allosteric modulator, restricting CP to a low-affinity, filament-binding conformation. Structure-based sequence alignments extend the CPI motif-containing family to include CIN85, CKIP-1, CapZIP and a relatively uncharacterized protein, WASHCAP (FAM21). Peptides comprising these CPI motifs are able to inhibit CP and to uncap CP-bound actin filaments.

Original languageEnglish
Pages (from-to)497-503
Number of pages7
JournalNature Structural and Molecular Biology
Volume17
Issue number4
DOIs
Publication statusPublished - Apr 1 2010
Externally publishedYes

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Protein Interaction Domains and Motifs
Actin Cytoskeleton
Actin Capping Proteins
Proteins
Agaricales
Actins
Sequence Alignment
Endocytosis
Polymerization
Cell Movement
Peptides

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Structural characterization of a capping protein interaction motif defines a family of actin filament regulators. / Hernandez-Valladares, Maria; Kim, Taekyung; Kannan, Balakrishnan; Tung, Alvin; Aguda, Adeleke H.; Larsson, Mrten; Cooper, John A.; Robinson, Robert C.

In: Nature Structural and Molecular Biology, Vol. 17, No. 4, 01.04.2010, p. 497-503.

Research output: Contribution to journalArticle

Hernandez-Valladares, Maria ; Kim, Taekyung ; Kannan, Balakrishnan ; Tung, Alvin ; Aguda, Adeleke H. ; Larsson, Mrten ; Cooper, John A. ; Robinson, Robert C. / Structural characterization of a capping protein interaction motif defines a family of actin filament regulators. In: Nature Structural and Molecular Biology. 2010 ; Vol. 17, No. 4. pp. 497-503.
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