Structural change of tRNA (Gm18) methyltransferase by binding of methyl donor analogues.

Kazunori Watanabe, Osamu Nureki, Shuya Fukai, Yaeta Endo, Hiroyuki Hori

Research output: Contribution to journalArticle

Abstract

Transfer RNA (Gm18) methyltransferase (TrmH, EC 2.1.1.34) catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH of the ribose of guanosine at position 18 in the D-loop of tRNA. The methyl group donor, AdoMet, is converted to S-adenosyl-L-homocysteine (AdoHcy) in the reaction. Recently, we determined the crystal structures of the AdoMet-bound and apo-forms of TrmH from Thermus thermophilus HB8. Based on the crystal structures, we carried out site-directed mutagenesis to clarify the functions of the conserved amino acid residues among SpoU RNA methyltransferases. During the course of the study, we found that the subunit structure of the wild-type TrmH changed according to the presence or absence of AdoMet analogues. In the absence of AdoMet or AdoHcy, the enzyme forms a homodimer. However, the addition of AdoMet induced the formation of a tetramer structure. Furthermore, the addition of AdoHcy caused the dissociation of subunits and gave rise to a monomer structure. These findings suggest that the dissociation of tRNA from the tRNA-enzyme complex after the methyl-transfer reaction is caused by the dissociation of enzyme subunits. In this meeting, we discuss the relationship between the catalytic mechanism and the change of the subunit structure of TrmH.

Original languageEnglish
Pages (from-to)301-302
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number49
Publication statusPublished - 2005
Externally publishedYes

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tRNA Methyltransferases
Methionine
Homocysteine
Transfer RNA
Enzymes
Thermus thermophilus
S-Adenosylmethionine
Ribose
Guanosine
Methyltransferases
Site-Directed Mutagenesis
RNA
Amino Acids

ASJC Scopus subject areas

  • Medicine(all)

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Structural change of tRNA (Gm18) methyltransferase by binding of methyl donor analogues. / Watanabe, Kazunori; Nureki, Osamu; Fukai, Shuya; Endo, Yaeta; Hori, Hiroyuki.

In: Nucleic acids symposium series (2004), No. 49, 2005, p. 301-302.

Research output: Contribution to journalArticle

Watanabe, Kazunori ; Nureki, Osamu ; Fukai, Shuya ; Endo, Yaeta ; Hori, Hiroyuki. / Structural change of tRNA (Gm18) methyltransferase by binding of methyl donor analogues. In: Nucleic acids symposium series (2004). 2005 ; No. 49. pp. 301-302.
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