Structural basis for perception of diverse chemical substances by T1r taste receptors

Nipawan Nuemket, Norihisa Yasui, Yuko Kusakabe, Yukiyo Nomura, Nanako Atsumi, Shuji Akiyama, Eriko Nango, Yukinari Kato, Mika K. Kaneko, Junichi Takagi, Maiko Hosotani, Atsuko Yamashita

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The taste receptor type 1 (T1r) family perceives 'palatable' tastes. These receptors function as T1r2-T1r3 and T1r1-T1r3 heterodimers to recognize a wide array of sweet and umami (savory) tastes in sugars and amino acids. Nonetheless, it is unclear how diverse tastes are recognized by so few receptors. Here we present crystal structures of the extracellular ligand-binding domains (LBDs), the taste recognition regions of the fish T1r2-T1r3 heterodimer, bound to different amino acids. The ligand-binding pocket in T1r2LBD is rich in aromatic residues, spacious and accommodates hydrated percepts. Biophysical studies show that this binding site is characterized by a broad yet discriminating chemical recognition, contributing for the particular trait of taste perception. In contrast, the analogous pocket in T1r3LBD is occupied by a rather loosely bound amino acid, suggesting that the T1r3 has an auxiliary role. Overall, we provide a structural basis for understanding the chemical perception of taste receptors.

Original languageEnglish
Article number15530
JournalNature Communications
Volume8
DOIs
Publication statusPublished - May 23 2017

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taste
Taste Perception
Amino Acids
Ligands
Sugar Acids
amino acids
Satureja
Fish
Crystal structure
Binding Sites
Fishes
ligands
fishes
sugars
acids
crystal structure
Recognition (Psychology)

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Structural basis for perception of diverse chemical substances by T1r taste receptors. / Nuemket, Nipawan; Yasui, Norihisa; Kusakabe, Yuko; Nomura, Yukiyo; Atsumi, Nanako; Akiyama, Shuji; Nango, Eriko; Kato, Yukinari; Kaneko, Mika K.; Takagi, Junichi; Hosotani, Maiko; Yamashita, Atsuko.

In: Nature Communications, Vol. 8, 15530, 23.05.2017.

Research output: Contribution to journalArticle

Nuemket, N, Yasui, N, Kusakabe, Y, Nomura, Y, Atsumi, N, Akiyama, S, Nango, E, Kato, Y, Kaneko, MK, Takagi, J, Hosotani, M & Yamashita, A 2017, 'Structural basis for perception of diverse chemical substances by T1r taste receptors', Nature Communications, vol. 8, 15530. https://doi.org/10.1038/ncomms15530
Nuemket, Nipawan ; Yasui, Norihisa ; Kusakabe, Yuko ; Nomura, Yukiyo ; Atsumi, Nanako ; Akiyama, Shuji ; Nango, Eriko ; Kato, Yukinari ; Kaneko, Mika K. ; Takagi, Junichi ; Hosotani, Maiko ; Yamashita, Atsuko. / Structural basis for perception of diverse chemical substances by T1r taste receptors. In: Nature Communications. 2017 ; Vol. 8.
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