Structural and functional roles of the N- and C-terminal extended modules in channelrhodopsin-1

Satoko Doi, Arisa Mori, Takashi Tsukamoto, Louisa Reissig, Kunio Ihara, Yuki Sudo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Channelrhodopsins have become a focus of interest because of their ability to control neural activity by light, used in a technology called optogenetics. The channelrhodopsin in the eukaryote Chlamydomonas reinhardtii (CrChR-1) is a light-gated cation channel responsible for motility changes upon photo-illumination and a member of the membrane-embedded retinal protein family. Recent crystal structure analysis revealed that CrChR-1 has unique extended modules both at its N- and C-termini compared to other microbial retinal proteins. This study reports the first successful expression of a ChR-1 variant in Escherichia coli as a holoprotein: the ChR-1 variant lacking both the N- and C-termini (CrChR-1-82-308). However, compared to ChR-1 having the extended modules (CrChR-1-1-357), truncation of the termini greatly altered the absorption maximum and photochemical properties, including the pKa values of its charged residues around the chromophore, the reaction rates in the photocycle and the photo-induced ion channeling activity. The results of some experiments regarding ion transport activity suggest that CrChR-1-82-308 has a proton channeling activity even in the dark. On the basis of these results, we discuss the structural and functional roles of the N- and C-terminal extended modules in CrChR-1.

Original languageEnglish
Pages (from-to)1628-1636
Number of pages9
JournalPhotochemical and Photobiological Sciences
Volume14
Issue number9
DOIs
Publication statusPublished - Jun 4 2015

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modules
Ions
Chromophores
Escherichia coli
Reaction rates
Cations
Protons
Proteins
Lighting
Crystal structure
eukaryotes
proteins
Membranes
locomotion
Escherichia
chromophores
ions
reaction kinetics
Experiments
illumination

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Structural and functional roles of the N- and C-terminal extended modules in channelrhodopsin-1. / Doi, Satoko; Mori, Arisa; Tsukamoto, Takashi; Reissig, Louisa; Ihara, Kunio; Sudo, Yuki.

In: Photochemical and Photobiological Sciences, Vol. 14, No. 9, 04.06.2015, p. 1628-1636.

Research output: Contribution to journalArticle

Doi, Satoko ; Mori, Arisa ; Tsukamoto, Takashi ; Reissig, Louisa ; Ihara, Kunio ; Sudo, Yuki. / Structural and functional roles of the N- and C-terminal extended modules in channelrhodopsin-1. In: Photochemical and Photobiological Sciences. 2015 ; Vol. 14, No. 9. pp. 1628-1636.
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