Abstract
We cloned and sequenced two vitellogenin (vg) cDNAs of the carp, Cyprinus carpio, using a cDNA library constructed from estradiol-17β (E2)-treated livers. One was a novel, longer 5000 bp-long cDNA termed vg-B2 encoding 1624 amino acids in a single open reading frame. The other was a shorter cDNA (vg-B1), identical to that registered previously as carp vg cDNA in the international nucleotide sequence database. The deduced amino acid sequences of these two molecules were well-aligned with known vertebrate Vgs sharing common characteristics such as N-terminal lipovitellin I (LVI), phosvitin (PV) and C-terminal lipovitellin II (LVII). The novel Vg-B2 bore a highly conserved GL/ICG motif within the LVII region, in contrast to the shorter Vg-B1 that has a truncated C-terminal and lacks the β-component within the LVII region including the GL/ICG motif. Both vg-B2 and vg-B1 genes were expressed in the livers of females and E2-injected males. Western blot analysis using anti-Vg and anti-vitellin (Vn) antisera demonstrated that both Vg-B2 and Vg-B1 were detected as polypeptides with an estimated molecular mass of 180 kDa and 160 kDa, respectively, in the blood of females and E2-injected males. The results suggest the potential utilization of these genes as sensitive xenoestrogenic markers.
| Original language | English |
|---|---|
| Pages (from-to) | 445-453 |
| Number of pages | 9 |
| Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| Volume | 148 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 2007 |
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Keywords
- Biomarker
- Carp
- Cyprinus carpio
- Estradiol-17β (E)
- Vitellogenin
ASJC Scopus subject areas
- Biochemistry
- Physiology
Cite this
Structural and expression analyses of two vitellogenin genes in the carp, Cyprinus carpio. / Kang, B. J.; Jung, J. H.; Lee, J. M.; Lim, S. G.; Saito, H.; Kim, M. H.; Kim, Y. J.; Saigusa, M.; Han, C. H.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 148, No. 4, 12.2007, p. 445-453.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structural and expression analyses of two vitellogenin genes in the carp, Cyprinus carpio
AU - Kang, B. J.
AU - Jung, J. H.
AU - Lee, J. M.
AU - Lim, S. G.
AU - Saito, H.
AU - Kim, M. H.
AU - Kim, Y. J.
AU - Saigusa, M.
AU - Han, C. H.
PY - 2007/12
Y1 - 2007/12
N2 - We cloned and sequenced two vitellogenin (vg) cDNAs of the carp, Cyprinus carpio, using a cDNA library constructed from estradiol-17β (E2)-treated livers. One was a novel, longer 5000 bp-long cDNA termed vg-B2 encoding 1624 amino acids in a single open reading frame. The other was a shorter cDNA (vg-B1), identical to that registered previously as carp vg cDNA in the international nucleotide sequence database. The deduced amino acid sequences of these two molecules were well-aligned with known vertebrate Vgs sharing common characteristics such as N-terminal lipovitellin I (LVI), phosvitin (PV) and C-terminal lipovitellin II (LVII). The novel Vg-B2 bore a highly conserved GL/ICG motif within the LVII region, in contrast to the shorter Vg-B1 that has a truncated C-terminal and lacks the β-component within the LVII region including the GL/ICG motif. Both vg-B2 and vg-B1 genes were expressed in the livers of females and E2-injected males. Western blot analysis using anti-Vg and anti-vitellin (Vn) antisera demonstrated that both Vg-B2 and Vg-B1 were detected as polypeptides with an estimated molecular mass of 180 kDa and 160 kDa, respectively, in the blood of females and E2-injected males. The results suggest the potential utilization of these genes as sensitive xenoestrogenic markers.
AB - We cloned and sequenced two vitellogenin (vg) cDNAs of the carp, Cyprinus carpio, using a cDNA library constructed from estradiol-17β (E2)-treated livers. One was a novel, longer 5000 bp-long cDNA termed vg-B2 encoding 1624 amino acids in a single open reading frame. The other was a shorter cDNA (vg-B1), identical to that registered previously as carp vg cDNA in the international nucleotide sequence database. The deduced amino acid sequences of these two molecules were well-aligned with known vertebrate Vgs sharing common characteristics such as N-terminal lipovitellin I (LVI), phosvitin (PV) and C-terminal lipovitellin II (LVII). The novel Vg-B2 bore a highly conserved GL/ICG motif within the LVII region, in contrast to the shorter Vg-B1 that has a truncated C-terminal and lacks the β-component within the LVII region including the GL/ICG motif. Both vg-B2 and vg-B1 genes were expressed in the livers of females and E2-injected males. Western blot analysis using anti-Vg and anti-vitellin (Vn) antisera demonstrated that both Vg-B2 and Vg-B1 were detected as polypeptides with an estimated molecular mass of 180 kDa and 160 kDa, respectively, in the blood of females and E2-injected males. The results suggest the potential utilization of these genes as sensitive xenoestrogenic markers.
KW - Biomarker
KW - Carp
KW - Cyprinus carpio
KW - Estradiol-17β (E)
KW - Vitellogenin
UR - http://www.scopus.com/inward/record.url?scp=35649003115&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35649003115&partnerID=8YFLogxK
U2 - 10.1016/j.cbpb.2007.07.088
DO - 10.1016/j.cbpb.2007.07.088
M3 - Article
C2 - 17804271
AN - SCOPUS:35649003115
VL - 148
SP - 445
EP - 453
JO - Comparative biochemistry and physiology. B, Comparative biochemistry
JF - Comparative biochemistry and physiology. B, Comparative biochemistry
SN - 1096-4959
IS - 4
ER -