Abstract
We cloned and sequenced two vitellogenin (vg) cDNAs of the carp, Cyprinus carpio, using a cDNA library constructed from estradiol-17β (E2)-treated livers. One was a novel, longer 5000 bp-long cDNA termed vg-B2 encoding 1624 amino acids in a single open reading frame. The other was a shorter cDNA (vg-B1), identical to that registered previously as carp vg cDNA in the international nucleotide sequence database. The deduced amino acid sequences of these two molecules were well-aligned with known vertebrate Vgs sharing common characteristics such as N-terminal lipovitellin I (LVI), phosvitin (PV) and C-terminal lipovitellin II (LVII). The novel Vg-B2 bore a highly conserved GL/ICG motif within the LVII region, in contrast to the shorter Vg-B1 that has a truncated C-terminal and lacks the β-component within the LVII region including the GL/ICG motif. Both vg-B2 and vg-B1 genes were expressed in the livers of females and E2-injected males. Western blot analysis using anti-Vg and anti-vitellin (Vn) antisera demonstrated that both Vg-B2 and Vg-B1 were detected as polypeptides with an estimated molecular mass of 180 kDa and 160 kDa, respectively, in the blood of females and E2-injected males. The results suggest the potential utilization of these genes as sensitive xenoestrogenic markers.
Original language | English |
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Pages (from-to) | 445-453 |
Number of pages | 9 |
Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
Volume | 148 |
Issue number | 4 |
DOIs | |
Publication status | Published - Dec 2007 |
Keywords
- Biomarker
- Carp
- Cyprinus carpio
- Estradiol-17β (E)
- Vitellogenin
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Aquatic Science
- Animal Science and Zoology
- Molecular Biology