Structural and expression analyses of two vitellogenin genes in the carp, Cyprinus carpio

B. J. Kang, J. H. Jung, J. M. Lee, S. G. Lim, H. Saito, M. H. Kim, Y. J. Kim, M. Saigusa, C. H. Han

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11 Citations (Scopus)

Abstract

We cloned and sequenced two vitellogenin (vg) cDNAs of the carp, Cyprinus carpio, using a cDNA library constructed from estradiol-17β (E2)-treated livers. One was a novel, longer 5000 bp-long cDNA termed vg-B2 encoding 1624 amino acids in a single open reading frame. The other was a shorter cDNA (vg-B1), identical to that registered previously as carp vg cDNA in the international nucleotide sequence database. The deduced amino acid sequences of these two molecules were well-aligned with known vertebrate Vgs sharing common characteristics such as N-terminal lipovitellin I (LVI), phosvitin (PV) and C-terminal lipovitellin II (LVII). The novel Vg-B2 bore a highly conserved GL/ICG motif within the LVII region, in contrast to the shorter Vg-B1 that has a truncated C-terminal and lacks the β-component within the LVII region including the GL/ICG motif. Both vg-B2 and vg-B1 genes were expressed in the livers of females and E2-injected males. Western blot analysis using anti-Vg and anti-vitellin (Vn) antisera demonstrated that both Vg-B2 and Vg-B1 were detected as polypeptides with an estimated molecular mass of 180 kDa and 160 kDa, respectively, in the blood of females and E2-injected males. The results suggest the potential utilization of these genes as sensitive xenoestrogenic markers.

Original languageEnglish
Pages (from-to)445-453
Number of pages9
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume148
Issue number4
DOIs
Publication statusPublished - Dec 2007

Keywords

  • Biomarker
  • Carp
  • Cyprinus carpio
  • Estradiol-17β (E)
  • Vitellogenin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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    Kang, B. J., Jung, J. H., Lee, J. M., Lim, S. G., Saito, H., Kim, M. H., Kim, Y. J., Saigusa, M., & Han, C. H. (2007). Structural and expression analyses of two vitellogenin genes in the carp, Cyprinus carpio. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 148(4), 445-453. https://doi.org/10.1016/j.cbpb.2007.07.088