Structural analysis of the L-methionine γ-lyase gene from Pseudomonas putida

Hiroyuki Inoue, Kenji Inagaki, Manabu Sugimoto, Nobuyoshi Esaki, Kenji Soda, Hidehiko Tanaka

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The gene encoding L-methionine γ-lyase from Pseudomonas putida was cloned and the primary structure of the enzyme was deduced from its nucleotide sequence. The L-methionine γ-lyase gene was expressed in Escherichia coli. The amino acid sequences of BrCN-digested peptides agreed with the corresponding parts of the L-methionine γ-lyase sequence determined from the gene structure. The polypeptide is composed of 398 amino acid residues with a calculated molecular weight of 42,626, corresponding to the subunit of the homotetrameric enzyme. The deduced amino acid sequence of L-methionine γ-lyase only showed extensive homology with other well known α,γ-elimination and/or γ-replacement pyridoxal 5'-phosphate-dependent enzymes, such as cystathionine γ-lyase, cystathionine γ-synthase, and O-acetylhomoserine O-acetylserine sulfhydrylase, that participate in the biosynthesis of sulfur amino acids. However, the deduced essential cysteine residue of L-methionine γ-lyase was not conserved in these enzymes. We confirmed the presence of a part of an open reading frame in the 3'-flanking region of the L-methionine γ-lyase gene, which showed high homology with the N-tenninal region of pyruvate dehydrogenase (lipoamide) from E. coli, suggesting that it participates in the degradative pathway for L-methionine together with L-methionine γ-lyase.

Original languageEnglish
Pages (from-to)1120-1125
Number of pages6
JournalJournal of Biochemistry
Volume117
Issue number5
Publication statusPublished - May 1995

Fingerprint

Pseudomonas putida
Lyases
Structural Analysis
Structural analysis
Methionine
Amino acids
Enzymes
Genes
Gene
Amino Acid Sequence
Escherichia coli
Escherichia Coli
Amino Acids
Homology
Cystathionine
Gene encoding
Biosynthesis
Polypeptides
Nucleotides
Phosphate

Keywords

  • L-methionine γ-lyase
  • Pseudomonas putida
  • Pyridoxal-P enzyme
  • Pyruvate dehydrogenase (lypoamide)

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Structural analysis of the L-methionine γ-lyase gene from Pseudomonas putida. / Inoue, Hiroyuki; Inagaki, Kenji; Sugimoto, Manabu; Esaki, Nobuyoshi; Soda, Kenji; Tanaka, Hidehiko.

In: Journal of Biochemistry, Vol. 117, No. 5, 05.1995, p. 1120-1125.

Research output: Contribution to journalArticle

Inoue, Hiroyuki ; Inagaki, Kenji ; Sugimoto, Manabu ; Esaki, Nobuyoshi ; Soda, Kenji ; Tanaka, Hidehiko. / Structural analysis of the L-methionine γ-lyase gene from Pseudomonas putida. In: Journal of Biochemistry. 1995 ; Vol. 117, No. 5. pp. 1120-1125.
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