Structural analysis of N-glycans of storage glycoproteins in soybean (glycine max. L) seed

Yoshinobu Kimura, Akira Ohno, Shigeaki Takagi

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

The structures of N-linked sugar chains (N-glycans) of storage glycoproteins in soybean seeds have been identified. Eight pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the storage glycoproteins by reverse-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were first identified by two-dimensional PA-sugar chain mapping and ion-spray mass analysis, considering the results of sugar composition analysis or sequential exoglycosidase digestion. The deduced structures were further analyzed by ion-spray tandem mass spectrometry and 500 MHz1H-NMR spectrometry. The eight structures fell into two categories; the major class (96.6%) was a typical high mannose-type, the minor class was a xylose containing-type (Man3Xyl1GlcNAc2, Man3Fuc1Xyl1GlcNAc2; 3.4%).

Original languageEnglish
Pages (from-to)1866-1871
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number11
DOIs
Publication statusPublished - Jan 1997

Keywords

  • Allergenic oligosaccharide
  • Glycine max
  • Ion-spray mass spectrometry
  • N-glycan
  • Plant glycoprotein

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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