Structural analysis of N-glycans of storage glycoproteins in soybean (glycine max. L) seed

Yoshinobu Kimura; Akira Ohno; Shigeaki Takagi

doi:10.1271/bbb.61.1866

Structural analysis of N-glycans of storage glycoproteins in soybean (glycine max. L) seed

Yoshinobu Kimura, Akira Ohno, Shigeaki Takagi

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

The structures of N-linked sugar chains (N-glycans) of storage glycoproteins in soybean seeds have been identified. Eight pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the storage glycoproteins by reverse-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were first identified by two-dimensional PA-sugar chain mapping and ion-spray mass analysis, considering the results of sugar composition analysis or sequential exoglycosidase digestion. The deduced structures were further analyzed by ion-spray tandem mass spectrometry and 500 MHz¹H-NMR spectrometry. The eight structures fell into two categories; the major class (96.6%) was a typical high mannose-type, the minor class was a xylose containing-type (Man₃Xyl₁GlcNAc₂, Man₃Fuc₁Xyl₁GlcNAc₂; 3.4%).

Original language	English
Pages (from-to)	1866-1871
Number of pages	6
Journal	Bioscience, Biotechnology and Biochemistry
Volume	61
Issue number	11
DOIs	https://doi.org/10.1271/bbb.61.1866
Publication status	Published - Jan 1997

Keywords

Allergenic oligosaccharide
Glycine max
Ion-spray mass spectrometry
N-glycan
Plant glycoprotein

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Structural analysis of N-glycans of storage glycoproteins in soybean (glycine max. L) seed",

abstract = "The structures of N-linked sugar chains (N-glycans) of storage glycoproteins in soybean seeds have been identified. Eight pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the storage glycoproteins by reverse-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were first identified by two-dimensional PA-sugar chain mapping and ion-spray mass analysis, considering the results of sugar composition analysis or sequential exoglycosidase digestion. The deduced structures were further analyzed by ion-spray tandem mass spectrometry and 500 MHz1H-NMR spectrometry. The eight structures fell into two categories; the major class (96.6%) was a typical high mannose-type, the minor class was a xylose containing-type (Man3Xyl1GlcNAc2, Man3Fuc1Xyl1GlcNAc2; 3.4%).",

keywords = "Allergenic oligosaccharide, Glycine max, Ion-spray mass spectrometry, N-glycan, Plant glycoprotein",

author = "Yoshinobu Kimura and Akira Ohno and Shigeaki Takagi",

note = "Funding Information: t This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, and Culture of Japan (No. 09660358) and from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Glyco-technology Project, No. 3322). tt To whom all correspondences should be addressed.",

year = "1997",

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doi = "10.1271/bbb.61.1866",

language = "English",

volume = "61",

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AU - Kimura, Yoshinobu

AU - Ohno, Akira

AU - Takagi, Shigeaki

N1 - Funding Information: t This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, and Culture of Japan (No. 09660358) and from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Glyco-technology Project, No. 3322). tt To whom all correspondences should be addressed.

PY - 1997/1

Y1 - 1997/1

N2 - The structures of N-linked sugar chains (N-glycans) of storage glycoproteins in soybean seeds have been identified. Eight pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the storage glycoproteins by reverse-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were first identified by two-dimensional PA-sugar chain mapping and ion-spray mass analysis, considering the results of sugar composition analysis or sequential exoglycosidase digestion. The deduced structures were further analyzed by ion-spray tandem mass spectrometry and 500 MHz1H-NMR spectrometry. The eight structures fell into two categories; the major class (96.6%) was a typical high mannose-type, the minor class was a xylose containing-type (Man3Xyl1GlcNAc2, Man3Fuc1Xyl1GlcNAc2; 3.4%).

AB - The structures of N-linked sugar chains (N-glycans) of storage glycoproteins in soybean seeds have been identified. Eight pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the storage glycoproteins by reverse-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were first identified by two-dimensional PA-sugar chain mapping and ion-spray mass analysis, considering the results of sugar composition analysis or sequential exoglycosidase digestion. The deduced structures were further analyzed by ion-spray tandem mass spectrometry and 500 MHz1H-NMR spectrometry. The eight structures fell into two categories; the major class (96.6%) was a typical high mannose-type, the minor class was a xylose containing-type (Man3Xyl1GlcNAc2, Man3Fuc1Xyl1GlcNAc2; 3.4%).

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KW - Ion-spray mass spectrometry

KW - N-glycan

KW - Plant glycoprotein

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Structural analysis of N-glycans of storage glycoproteins in soybean (glycine max. L) seed

Abstract

Keywords

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