Structural Analyses of Sugar Chains from Ricin A-Chain Variant

Yoshinobu Kimura, Hironobu Kusuoku, Mikiro Tada, Shigeaki Takagi, Gunki Funatsu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Two glycopeptides were isolated from a tryptic digest of ricin A-chain variant by gel filtration and reversed-phase HPLC. Their amino acid compositions and sequences showed that Asn-10 and Asn-236 are glycosylated. The sugar chains were liberated from these glycopeptides by hydrazynolysis, N-acetylated, and pyridylaminated. Component analysis and comparison of elution positions of the purified pyridylamino (PA-)sugar chains with those of authentic PA-sugar chains from the major ricin A-chain by reversed-phase HPLC and size fractionation HPLC suggested that both sugar chains linked to Asn-10 and Asn-236 of the ricin A-chain variant are M3FX, identical to that linked to Asn-10 of the major ricin A-chain.

Original languageEnglish
Pages (from-to)157-162
Number of pages6
JournalAgricultural and Biological Chemistry
Volume54
Issue number1
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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