Structural analyses of sugar chains from Abrus precatorius agglutinin

Yoshinobu Kimura, Sumihiro Hase, Tokuji Ikenaka, Gunki Funatsu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The structures of asparagine-linked sugar chains of Abrus precatorius agglutinin were determined. The sugar chains were liberated from the lectin by hydrazinolysis. After N-acetylation, the reducing-end residues of the sugar chains were coupled with 2-aminopyridine. The pyridylamino (PA-) derivatives thus obtained were purified by gel filtration and HPLC. The structures of the purified derivatives were identified by component analysis, comparison of their elution positions on HPLC with those of PA-sugar chains of known structures, stepwise exoglycosidase digestion, and partial acetolysis. The major four surgar chains obtained from A. precatorius agglutinin were Manα1 → 2Manα1 → 6(Manα1 → 3)Manβ1 → 6(Manα1 → 2Manα2 → 2Manα1 → 3)Manα1 → GlcNAcβ1 → GlcNAc-PA, Manα1 → 2Manα1 → 6(Manα1 → 3)Manα1 → 6(Manα1 → 2Manα1 →3)Manβ1 → GlcNAcβ1 → GlcNAc-PA, Manα1 → 6(Manα1 → 3)Manα1 → 6(Manα1 → 2Manα1 → 3)Manβ1 → 4GlcNAcβ1 → 4GlcNAc-PA, and Manα1 → 6(Manα1 → 34) (Xylβ1 → 2)Manβ1 → 4GlcNAcβ1 → 4(Fucα1 → 3)GlcNAc-PA.

Original languageEnglish
Pages (from-to)160-167
Number of pages8
JournalBBA - General Subjects
Volume966
Issue number1
DOIs
Publication statusPublished - Jul 14 1988

Keywords

  • (A. precatorius)
  • Agglutinin
  • Glycoprotein
  • Lectin
  • Pyridylamino derivative
  • Sugar chain processing
  • Sugar chain structure

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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