Stromal cells promote bone invasion by suppressing bone formation in ameloblastoma

G. S A Sathi, Hitoshi Nagatsuka, R. Tamamura, M. Fujii, M. Gunduz, M. Inoue, R. S. Rivera, N. Nagai

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aims: To study the stromal variation and role of stromal-tumour cell interaction in impaired bone formation as well as enhanced bone resorption in ameloblastoma. Methods and results: Four types of stroma were observed histologically; fibrous, desmoplastic, myxoid and myxoid with hyalinization. Osteoblast and osteoclast were counted using haematoxylin and eosin sections and immunohistochemistry with CD68. After histomorphometric analysis, only fibrous and myxoid types of stroma were distinctly identified. Secreted frizzled-related peptide (sFRP)-2, transforming growth factor-beta 1 and receptor activator of nuclear factor-κB ligand (RANKL) revealed strong expression in myxoid type compared with the normal stroma. Bone morphogenetic protein (BMP)-2 was negative in myxoid type, but positive in normal stroma. Fibrous-type stroma showed weak expression of all antigens except RANKL compared with myxoid type. Conclusions: The results suggest that stroma does not act only in bone resorption, but also in the suppression of new bone formation. sFRP-2 is the main factor for impaired bone formation. The expression of markers related to osteoclastogenesis and suppression of osteoblast formation is higher in myxoid-type than in fibrous-type stroma. Tumour cells create a favourable environment for impaired bone formation by secreting sFRP-2 as well as bone resorption by secreting RANKL and interleukin-6.

Original languageEnglish
Pages (from-to)458-467
Number of pages10
JournalHistopathology
Volume53
Issue number4
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Ameloblastoma
Stromal Cells
Osteogenesis
Bone and Bones
Bone Resorption
Osteoblasts
Peptides
Bone Morphogenetic Protein 2
Osteoclasts
Hematoxylin
Eosine Yellowish-(YS)
Cell Communication
Interleukin-6
Neoplasms
Immunohistochemistry
Ligands
Antigens

Keywords

  • Ameloblastoma
  • Osteoblast
  • Osteoclast
  • SFRP-2
  • Stroma

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Sathi, G. S. A., Nagatsuka, H., Tamamura, R., Fujii, M., Gunduz, M., Inoue, M., ... Nagai, N. (2008). Stromal cells promote bone invasion by suppressing bone formation in ameloblastoma. Histopathology, 53(4), 458-467. https://doi.org/10.1111/j.1365-2559.2008.03127.x

Stromal cells promote bone invasion by suppressing bone formation in ameloblastoma. / Sathi, G. S A; Nagatsuka, Hitoshi; Tamamura, R.; Fujii, M.; Gunduz, M.; Inoue, M.; Rivera, R. S.; Nagai, N.

In: Histopathology, Vol. 53, No. 4, 10.2008, p. 458-467.

Research output: Contribution to journalArticle

Sathi, GSA, Nagatsuka, H, Tamamura, R, Fujii, M, Gunduz, M, Inoue, M, Rivera, RS & Nagai, N 2008, 'Stromal cells promote bone invasion by suppressing bone formation in ameloblastoma', Histopathology, vol. 53, no. 4, pp. 458-467. https://doi.org/10.1111/j.1365-2559.2008.03127.x
Sathi, G. S A ; Nagatsuka, Hitoshi ; Tamamura, R. ; Fujii, M. ; Gunduz, M. ; Inoue, M. ; Rivera, R. S. ; Nagai, N. / Stromal cells promote bone invasion by suppressing bone formation in ameloblastoma. In: Histopathology. 2008 ; Vol. 53, No. 4. pp. 458-467.
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