Striatal stimulation Nurtures endogenous neurogenesis and angiogenesis in chronic-phase ischemic stroke rats

Takamasa Morimoto; Takao Yasuhara; Masahiro Kameda; Tanefumi Baba; Satoshi Kuramoto; Akihiko Kondo; Kazuya Takahashi; Naoki Tajiri; Feifei Wang; Jing Meng; Yuan Wen Ji; Tomohito Kadota; Tomoko Maruo; Kazushi Kinugasa; Yasuyuki Miyoshi; Tetsuro Shingo; Cesario V. Borlongan; Isao Date

doi:10.3727/096368910X544915

Striatal stimulation Nurtures endogenous neurogenesis and angiogenesis in chronic-phase ischemic stroke rats

Takamasa Morimoto, Takao Yasuhara, Masahiro Kameda, Tanefumi Baba, Satoshi Kuramoto, Akihiko Kondo, Kazuya Takahashi, Naoki Tajiri, Feifei Wang, Jing Meng, Yuan Wen Ji, Tomohito Kadota, Tomoko Maruo, Kazushi Kinugasa, Yasuyuki Miyoshi, Tetsuro Shingo, Cesario V. Borlongan, Isao Date

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Deep brain stimulation (DBS) is used to treat a variety of neurological disorders including Parkinson's disease. In this study, we explored the effects of striatal stimulation (SS) in a rat model of chronic-phase ischemic stroke. The stimulation electrode was implanted into the ischemic penumbra at 1 month after middle cerebral artery occlusion (MCAO) and thereafter continuously delivered SS over a period of 1 week. Rats were evaluated behaviorally coupled with neuroradiological assessment of the infarct volumes using magnetic resonance imaging (MRI) at pre-and post-SS. The rats with SS showed significant behavioral recovery in the spontaneous activity and limb placement test compared to those without SS. MRI visualized that SS also significantly reduced the infarct volumes compared to that at pre-SS or without SS. Immunohistochemical analyses revealed a robust neurogenic response in rats that received SS characterized by a stream of proliferating cells from the subventricular zone migrating to and subsequently differentiating into neurons in the ischemic penumbra, which exhibited a significant GDNF upregulation. In tandem with this SS-mediated neurogenesis, enhanced angiogenesis was also recognized as revealed by a significant increase in VEGF levels in the penumbra. These results provide evidence that SS affords neurorestoration at the chronic phase of stroke by stimulating endogenous neurogenesis and angiogenesis.

Original language	English
Pages (from-to)	1049-1064
Number of pages	16
Journal	Cell Transplantation
Volume	20
Issue number	7
DOIs	https://doi.org/10.3727/096368910X544915
Publication status	Published - 2011

Keywords

Angiogenesis
Chemoattractant
Electrical stimulation
Inflammation
Neural stem/progenitor cells
Neurogenesis

ASJC Scopus subject areas

Biomedical Engineering
Cell Biology
Transplantation

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Morimoto, T., Yasuhara, T., Kameda, M., Baba, T., Kuramoto, S., Kondo, A., Takahashi, K., Tajiri, N., Wang, F., Meng, J., Ji, Y. W., Kadota, T., Maruo, T., Kinugasa, K., Miyoshi, Y., Shingo, T., Borlongan, C. V., & Date, I. (2011). Striatal stimulation Nurtures endogenous neurogenesis and angiogenesis in chronic-phase ischemic stroke rats. Cell Transplantation, 20(7), 1049-1064. https://doi.org/10.3727/096368910X544915

Morimoto, T, Yasuhara, T, Kameda, M, Baba, T, Kuramoto, S, Kondo, A, Takahashi, K, Tajiri, N, Wang, F, Meng, J, Ji, YW, Kadota, T, Maruo, T, Kinugasa, K, Miyoshi, Y, Shingo, T, Borlongan, CV & Date, I 2011, 'Striatal stimulation Nurtures endogenous neurogenesis and angiogenesis in chronic-phase ischemic stroke rats', Cell Transplantation, vol. 20, no. 7, pp. 1049-1064. https://doi.org/10.3727/096368910X544915

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abstract = "Deep brain stimulation (DBS) is used to treat a variety of neurological disorders including Parkinson's disease. In this study, we explored the effects of striatal stimulation (SS) in a rat model of chronic-phase ischemic stroke. The stimulation electrode was implanted into the ischemic penumbra at 1 month after middle cerebral artery occlusion (MCAO) and thereafter continuously delivered SS over a period of 1 week. Rats were evaluated behaviorally coupled with neuroradiological assessment of the infarct volumes using magnetic resonance imaging (MRI) at pre-and post-SS. The rats with SS showed significant behavioral recovery in the spontaneous activity and limb placement test compared to those without SS. MRI visualized that SS also significantly reduced the infarct volumes compared to that at pre-SS or without SS. Immunohistochemical analyses revealed a robust neurogenic response in rats that received SS characterized by a stream of proliferating cells from the subventricular zone migrating to and subsequently differentiating into neurons in the ischemic penumbra, which exhibited a significant GDNF upregulation. In tandem with this SS-mediated neurogenesis, enhanced angiogenesis was also recognized as revealed by a significant increase in VEGF levels in the penumbra. These results provide evidence that SS affords neurorestoration at the chronic phase of stroke by stimulating endogenous neurogenesis and angiogenesis.",

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AU - Morimoto, Takamasa

AU - Yasuhara, Takao

AU - Kameda, Masahiro

AU - Baba, Tanefumi

AU - Kuramoto, Satoshi

AU - Kondo, Akihiko

AU - Takahashi, Kazuya

AU - Tajiri, Naoki

AU - Wang, Feifei

AU - Meng, Jing

AU - Ji, Yuan Wen

AU - Kadota, Tomohito

AU - Maruo, Tomoko

AU - Kinugasa, Kazushi

AU - Miyoshi, Yasuyuki

AU - Shingo, Tetsuro

AU - Borlongan, Cesario V.

AU - Date, Isao

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AB - Deep brain stimulation (DBS) is used to treat a variety of neurological disorders including Parkinson's disease. In this study, we explored the effects of striatal stimulation (SS) in a rat model of chronic-phase ischemic stroke. The stimulation electrode was implanted into the ischemic penumbra at 1 month after middle cerebral artery occlusion (MCAO) and thereafter continuously delivered SS over a period of 1 week. Rats were evaluated behaviorally coupled with neuroradiological assessment of the infarct volumes using magnetic resonance imaging (MRI) at pre-and post-SS. The rats with SS showed significant behavioral recovery in the spontaneous activity and limb placement test compared to those without SS. MRI visualized that SS also significantly reduced the infarct volumes compared to that at pre-SS or without SS. Immunohistochemical analyses revealed a robust neurogenic response in rats that received SS characterized by a stream of proliferating cells from the subventricular zone migrating to and subsequently differentiating into neurons in the ischemic penumbra, which exhibited a significant GDNF upregulation. In tandem with this SS-mediated neurogenesis, enhanced angiogenesis was also recognized as revealed by a significant increase in VEGF levels in the penumbra. These results provide evidence that SS affords neurorestoration at the chronic phase of stroke by stimulating endogenous neurogenesis and angiogenesis.

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Striatal stimulation Nurtures endogenous neurogenesis and angiogenesis in chronic-phase ischemic stroke rats

Abstract

Keywords

ASJC Scopus subject areas

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