Stretching of single DNA molecules caused by accelerating flow on a microchip

Ken Hirano; Takafumi Iwaki; Tomomi Ishido; Yuko Yoshikawa; Keiji Naruse; Kenichi Yoshikawa

doi:10.1063/1.5040564

Stretching of single DNA molecules caused by accelerating flow on a microchip

Ken Hirano, Takafumi Iwaki, Tomomi Ishido, Yuko Yoshikawa, Keiji Naruse, Kenichi Yoshikawa

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

DNA elongation induced by fluidic stress was investigated on a microfluidic chip composed of a large inlet pool and a narrow channel. Through single-DNA observation with fluorescence microscopy, the manner of stretching of individual T4 DNA molecules (166 kbp) was monitored near the area of accelerating flow with narrowing streamlines. The results showed that the DNA long-axis length increased in a sigmoidal manner depending on the magnitude of flow acceleration, or shear, along the DNA chain. To elucidate the physical mechanism of DNA elongation, we performed a theoretical study by adopting a model of a coarse-grained nonlinear elastic polymer chain elongated by shear stress due to acceleration flow along the chain direction.

Original language	English
Article number	165101
Journal	Journal of Chemical Physics
Volume	149
Issue number	16
DOIs	https://doi.org/10.1063/1.5040564
Publication status	Published - Oct 28 2018

ASJC Scopus subject areas

Physics and Astronomy(all)
Physical and Theoretical Chemistry

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title = "Stretching of single DNA molecules caused by accelerating flow on a microchip",

abstract = "DNA elongation induced by fluidic stress was investigated on a microfluidic chip composed of a large inlet pool and a narrow channel. Through single-DNA observation with fluorescence microscopy, the manner of stretching of individual T4 DNA molecules (166 kbp) was monitored near the area of accelerating flow with narrowing streamlines. The results showed that the DNA long-axis length increased in a sigmoidal manner depending on the magnitude of flow acceleration, or shear, along the DNA chain. To elucidate the physical mechanism of DNA elongation, we performed a theoretical study by adopting a model of a coarse-grained nonlinear elastic polymer chain elongated by shear stress due to acceleration flow along the chain direction.",

author = "Ken Hirano and Takafumi Iwaki and Tomomi Ishido and Yuko Yoshikawa and Keiji Naruse and Kenichi Yoshikawa",

note = "Funding Information: This work was supported by JSPS KAKENHI, Grant Nos. 25790030, 15H02121, 25103012, and 18K06175. Publisher Copyright: {\textcopyright} 2018 Author(s).",

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doi = "10.1063/1.5040564",

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T1 - Stretching of single DNA molecules caused by accelerating flow on a microchip

AU - Hirano, Ken

AU - Iwaki, Takafumi

AU - Ishido, Tomomi

AU - Yoshikawa, Yuko

AU - Naruse, Keiji

AU - Yoshikawa, Kenichi

PY - 2018/10/28

Y1 - 2018/10/28

N2 - DNA elongation induced by fluidic stress was investigated on a microfluidic chip composed of a large inlet pool and a narrow channel. Through single-DNA observation with fluorescence microscopy, the manner of stretching of individual T4 DNA molecules (166 kbp) was monitored near the area of accelerating flow with narrowing streamlines. The results showed that the DNA long-axis length increased in a sigmoidal manner depending on the magnitude of flow acceleration, or shear, along the DNA chain. To elucidate the physical mechanism of DNA elongation, we performed a theoretical study by adopting a model of a coarse-grained nonlinear elastic polymer chain elongated by shear stress due to acceleration flow along the chain direction.

AB - DNA elongation induced by fluidic stress was investigated on a microfluidic chip composed of a large inlet pool and a narrow channel. Through single-DNA observation with fluorescence microscopy, the manner of stretching of individual T4 DNA molecules (166 kbp) was monitored near the area of accelerating flow with narrowing streamlines. The results showed that the DNA long-axis length increased in a sigmoidal manner depending on the magnitude of flow acceleration, or shear, along the DNA chain. To elucidate the physical mechanism of DNA elongation, we performed a theoretical study by adopting a model of a coarse-grained nonlinear elastic polymer chain elongated by shear stress due to acceleration flow along the chain direction.

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Stretching of single DNA molecules caused by accelerating flow on a microchip

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