Stretch-induced IL-6 secretion from endothelial cells requires NF-κB activation

Satoshi Kobayashi, Masato Nagino, Shunichiro Komatsu, Keiji Naruse, Yuji Nimura, Makoto Nakanishi, Masahiro Sokabe

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Interleukin-6 (IL-6) secretion from endothelial cells (ECs) in response to mechanical stimuli plays an important role in the regenerative and inflammatory responses. The aim of this study was to determine the mechanism for the secretion of IL-6 from ECs in response to uni-axial continuous stretch. Continuous stretch induced IL-6 secretion from human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. Reverse transcriptase-polymerase chain reaction (RT-PCR) amplification showed that the transcription of the IL-6 gene peaked 2h after stretch. In vitro kinase assay of IκB kinase (IKKs) activity demonstrated that the activation of IKKs peaked 15min after stretch. Two NF-κB inhibitors, pyrrolidine dithiocarbamanate (PDTC) and SN50, or antisense oligodeoxynucleotides for NF-κB p65 and p50 suppressed IL-6 mRNA expressions induced by continuous stretch. In conclusion, continuous stretch induces IL-6 secretion from ECs, most likely through sequential activation of IKKs and NF-κB.

Original languageEnglish
Pages (from-to)306-312
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume308
Issue number2
DOIs
Publication statusPublished - Aug 22 2003
Externally publishedYes

Keywords

  • Continuous stretch
  • HUVECs
  • IL-6
  • NF-κB

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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