[Stress response genes expression analysis of barley Hordeum vulgare under space flight environment].

E. I. Shagimardanova, O. A. Gusev, V. N. Sychev, M. A. Levinskikh, M. R. Sharipova, O. N. Il'inskaia, G. Bingham, Manabu Sugimoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Transcriptome of barley Hordeum vulgare grown aboard International Space Station (ISS) was analyzed by means of microarray. It was revealed 500 genes with mRNA level, changed more than two folds in space environment. Among them are genes encoding stress response proteins, videlicet Heat Shock Proteins (HSP), Pathogenesis-Related Proteins (PR) and Antioxidant Proteins. Further analysis of these genes by real time PCR showed enhanced transcription level of Reactive oxygen Species (ROS) scavenging genes. The mRNA level of superoxide dismutase (sod) was 6 folds higher in space environment when compare to Earth conditions. Glutamyl transferase gene expression was enhanced 24 times in space. Transcription of catalase gene (cat) was increased 18 times and of ascorbate peroxidase was increased 3 times in space in comparison with ground control. For the first time it was shown that space flight environment may induce oxidative stress in plants.

Original languageEnglish
Pages (from-to)831-838
Number of pages8
JournalMolekuliarnaia biologiia
Volume44
Issue number5
Publication statusPublished - Sep 2010
Externally publishedYes

Fingerprint

Space Flight
Hordeum
Gene Expression
Genes
Heat-Shock Proteins
Ascorbate Peroxidases
Messenger RNA
Transferases
Transcriptome
Catalase
Superoxide Dismutase
Real-Time Polymerase Chain Reaction
Reactive Oxygen Species
Proteins
Oxidative Stress
Cats
Antioxidants

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Shagimardanova, E. I., Gusev, O. A., Sychev, V. N., Levinskikh, M. A., Sharipova, M. R., Il'inskaia, O. N., ... Sugimoto, M. (2010). [Stress response genes expression analysis of barley Hordeum vulgare under space flight environment]. Molekuliarnaia biologiia, 44(5), 831-838.

[Stress response genes expression analysis of barley Hordeum vulgare under space flight environment]. / Shagimardanova, E. I.; Gusev, O. A.; Sychev, V. N.; Levinskikh, M. A.; Sharipova, M. R.; Il'inskaia, O. N.; Bingham, G.; Sugimoto, Manabu.

In: Molekuliarnaia biologiia, Vol. 44, No. 5, 09.2010, p. 831-838.

Research output: Contribution to journalArticle

Shagimardanova, EI, Gusev, OA, Sychev, VN, Levinskikh, MA, Sharipova, MR, Il'inskaia, ON, Bingham, G & Sugimoto, M 2010, '[Stress response genes expression analysis of barley Hordeum vulgare under space flight environment].', Molekuliarnaia biologiia, vol. 44, no. 5, pp. 831-838.
Shagimardanova EI, Gusev OA, Sychev VN, Levinskikh MA, Sharipova MR, Il'inskaia ON et al. [Stress response genes expression analysis of barley Hordeum vulgare under space flight environment]. Molekuliarnaia biologiia. 2010 Sep;44(5):831-838.
Shagimardanova, E. I. ; Gusev, O. A. ; Sychev, V. N. ; Levinskikh, M. A. ; Sharipova, M. R. ; Il'inskaia, O. N. ; Bingham, G. ; Sugimoto, Manabu. / [Stress response genes expression analysis of barley Hordeum vulgare under space flight environment]. In: Molekuliarnaia biologiia. 2010 ; Vol. 44, No. 5. pp. 831-838.
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