Stress protein inductions after brain ischemia

Koji Abe, J. Kawagoe, M. Aoki, K. Kogure, Y. Itoyama

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

1. Hippocampal CA1 neurons are the most vulnerable to transient cerebral ischemia. However, the mechanism has not been fully understood. 2. The mRNAs for 72-kd (HSP72) and 73-kd (HSC73) heat shock proteins (HSPs), which are located mainly in the cytoplasm, were greatly induced together in CA1 cells, with a peak at 1-2 days in gerbils. However, immunoreactive HSP72 protein was only minimally expressed in CA1 neurons. 3. The mRNA for mitochondrial HSP60 began to increase at 3 hr in CA1 cells and was sustained until 1 day. 4. The level of mRNA for cytochrome c oxidase subunit I (COX-I) progressively decreased in CA1 neurons after a transient ischemia and completely disappeared at 7 days. The activity of cytochrome c oxidase (COX) protein also showed an early decrease in CA1 cells and was followed by a reduction in the level of COX-I DNA after 2 days. 5. These results suggest that HSP gene inductions were inhibited at the translational level but that mitochondrial DNA expression was disturbed at the transcriptional level. A disturbance of mitochondrial DNA expression could cause progressive failure of energy production of CA1 cells that eventually results in neuronal cell death.

Original languageEnglish
Pages (from-to)709-719
Number of pages11
JournalCellular and Molecular Neurobiology
Volume18
Issue number6
DOIs
Publication statusPublished - 1998

Fingerprint

Heat-Shock Proteins
Brain Ischemia
Neurons
Brain
Electron Transport Complex IV
Mitochondrial DNA
Messenger RNA
Cell death
Gerbillinae
Transient Ischemic Attack
Oxidoreductases
Proteins
Genes
Cytoplasm
Cell Death
Ischemia
DNA

Keywords

  • Cytochrome c oxidase
  • HSC73
  • HSP60
  • HSP72
  • Ischemia

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Biochemistry
  • Cell Biology
  • Genetics

Cite this

Stress protein inductions after brain ischemia. / Abe, Koji; Kawagoe, J.; Aoki, M.; Kogure, K.; Itoyama, Y.

In: Cellular and Molecular Neurobiology, Vol. 18, No. 6, 1998, p. 709-719.

Research output: Contribution to journalArticle

Abe, K, Kawagoe, J, Aoki, M, Kogure, K & Itoyama, Y 1998, 'Stress protein inductions after brain ischemia', Cellular and Molecular Neurobiology, vol. 18, no. 6, pp. 709-719. https://doi.org/10.1023/A:1020230220971
Abe, Koji ; Kawagoe, J. ; Aoki, M. ; Kogure, K. ; Itoyama, Y. / Stress protein inductions after brain ischemia. In: Cellular and Molecular Neurobiology. 1998 ; Vol. 18, No. 6. pp. 709-719.
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