Stress-Axis Regulated Exon (STREX) in the C terminus of BKCa channels is responsible for the stretch sensitivity

Keiji Naruse, Qiong Yao Tang, Masahiro Sokabe

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We previously reported that SAKCA, a stretch-activated, large-conductance, calcium- and voltage-activated potassium (BKCa) channel is present in chick embryonic heart. Here, we cloned SAKCA and identified that Stress-Axis Regulated Exon (STREX) is responsible for the stretch sensitivity. Single patch-clamp recordings from CHO cells transfected with the cloned SAKCA showed stretch sensitivity, whereas deletion of the STREX insert diminished the stretch sensitivity of the channel. Sequence analysis revealed that the ERA 672-674 sequence of the STREX is indispensable for channel stretch sensitivity and single amino acid substitution from Ala674 to Thr674 completely eliminated the stretch sensitivity. Co-expression of chick STREX-EGFP and SAKCA in CHO cells, induced a strong GFP signal in the cell membrane and inhibited the stretch sensitivity significantly. These results suggest that SAKCA senses membrane tension through an interaction between STREX and submembranous components.

Original languageEnglish
Pages (from-to)634-639
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume385
Issue number4
DOIs
Publication statusPublished - Aug 7 2009

Fingerprint

Exons
CHO Cells
Potassium Channels
Clamping devices
Amino Acid Substitution
Cell membranes
Sequence Analysis
Potassium
Substitution reactions
Cell Membrane
Calcium
Membranes
Amino Acids
Electric potential

Keywords

  • BK channel
  • Heart
  • Large-conductance K channel
  • SA channel
  • STREX

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Stress-Axis Regulated Exon (STREX) in the C terminus of BKCa channels is responsible for the stretch sensitivity. / Naruse, Keiji; Tang, Qiong Yao; Sokabe, Masahiro.

In: Biochemical and Biophysical Research Communications, Vol. 385, No. 4, 07.08.2009, p. 634-639.

Research output: Contribution to journalArticle

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AB - We previously reported that SAKCA, a stretch-activated, large-conductance, calcium- and voltage-activated potassium (BKCa) channel is present in chick embryonic heart. Here, we cloned SAKCA and identified that Stress-Axis Regulated Exon (STREX) is responsible for the stretch sensitivity. Single patch-clamp recordings from CHO cells transfected with the cloned SAKCA showed stretch sensitivity, whereas deletion of the STREX insert diminished the stretch sensitivity of the channel. Sequence analysis revealed that the ERA 672-674 sequence of the STREX is indispensable for channel stretch sensitivity and single amino acid substitution from Ala674 to Thr674 completely eliminated the stretch sensitivity. Co-expression of chick STREX-EGFP and SAKCA in CHO cells, induced a strong GFP signal in the cell membrane and inhibited the stretch sensitivity significantly. These results suggest that SAKCA senses membrane tension through an interaction between STREX and submembranous components.

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