Strain-dependent induction of allergic rhinitis without adjuvant in mice

D. A. Harn, M. Abe, M. M. Wang, A. R. Satoskar, Kazunori Nishizaki, Y. Masuda, Tadashi Yoshino, Mitsuhiro Okano

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Background: To date, no murine models have been reported to show the induction of both antigen-specific IgE and nasal eosinophilia, two of the major hallmarks of allergic rhinitis, after local sensitization in the absence of adjuvants, a phenomenon which reflects natural exposure. In this report, we attempted to establish a murine model representing an initiation of allergic rhinitis. Methods: BALB/c, CBA/J, and C57BL/6 mice were sensitized intranasally to Schistosoma mansoni egg antigen (SEA) solely. After repeated sensitization, serum Ab titers, nasal eosinophilia, and cytokine production by nasal lymphocytes were determined. Results: BALB/c mice produced SEA-specific IgE after repeated sensitization. High-dose sensitization to SEA induced IgE production in CBA/J mice, while C57BL/6 mice did not show the production throughout the period observed, suggesting that IgE production was regulated genetically. BALB/c mice also exhibited nasal eosinophilia after the nasal challenge. In addition, nasal lymphocytes sensitized with SEA intranasally produced significant amount of IL-5 in vitro. Conclusions: These results suggest that intranasal sensitization with SEA in the absence of adjuvants induces a Th2 immune reaction, reflecting the hallmarks of the initiation of allergic rhinitis both in vivo and in vitro, which is genetically regulated.

Original languageEnglish
Pages (from-to)593-601
Number of pages9
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume54
Issue number6
DOIs
Publication statusPublished - 1999

Fingerprint

Nose
Schistosoma mansoni
Ovum
Antigens
Immunoglobulin E
Eosinophilia
Inbred C57BL Mouse
Lymphocytes
Inbred CBA Mouse
Interleukin-5
Allergic Rhinitis
Cytokines
Serum

Keywords

  • Adjuvant
  • Allergic rhinitis
  • Cytokine
  • Eosinophilia
  • IgE
  • Intranasal sensitization
  • Mouse
  • Strain

ASJC Scopus subject areas

  • Immunology

Cite this

Strain-dependent induction of allergic rhinitis without adjuvant in mice. / Harn, D. A.; Abe, M.; Wang, M. M.; Satoskar, A. R.; Nishizaki, Kazunori; Masuda, Y.; Yoshino, Tadashi; Okano, Mitsuhiro.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 54, No. 6, 1999, p. 593-601.

Research output: Contribution to journalArticle

Harn, D. A. ; Abe, M. ; Wang, M. M. ; Satoskar, A. R. ; Nishizaki, Kazunori ; Masuda, Y. ; Yoshino, Tadashi ; Okano, Mitsuhiro. / Strain-dependent induction of allergic rhinitis without adjuvant in mice. In: Allergy: European Journal of Allergy and Clinical Immunology. 1999 ; Vol. 54, No. 6. pp. 593-601.
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