Stimulatory roles of muscarinic acetylcholine receptors on T cell antigen receptor/CD3 complex-mediated interleukin-2 production in human peripheral blood lymphocytes

Hiromichi Fujino, Yoshihisa Kitamura, Takeshi Yada, Takashi Uehara, Yasuyuki Nomura

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

It is known that there are some bidirectional interactions between the nervous and the immune systems via neurotransmitters and cytokines. To clarify whether any neurotransmitters modulate lymphocyte functions, we examined the effects of oxotremorine-M (Oxo-M) on interleukin-2 (IL-2) production in human peripheral blood lymphocytes by using enzyme-linked immunosorbent assays, Northern blot analyses, reverse transcriptase- polymerase chain reaction, and fluorescence-activated cell sorter. Pretreatment of cells with Oxo-M (10 nM to 10 μM) for 4-24 hr enhanced phytohemagglutinin (PHA)-induced IL-2 mRNA expression and markedly increased IL-2 production compared with those induced by PHA alone. Oxo-M alone did not affect IL-2 mRNA expression and IL-2 production. In CD3-positive T cells, pretreatment with Oxo-M for 24 hr enhanced PHA-induced IL-2 production. Furthermore, pretreatment with Oxo-M enhanced PHA-induced mRNA expression of the α and β subunits of IL-2 receptors and DNA synthesis. Cytometric analysis showed Oxo-M treatment did not up-regulate expression of cell surface molecules such as CD3, CD2, CD4, CD8, and IL-2 receptors. These results suggest that activation of muscarinic receptors enhances T cell antigen receptor/CD3-induced IL-2 production.

Original languageEnglish
Pages (from-to)1007-1014
Number of pages8
JournalMolecular Pharmacology
Volume51
Issue number6
Publication statusPublished - Jun 1997
Externally publishedYes

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T-Cell Antigen Receptor-CD3 Complex
Muscarinic Receptors
Interleukin-2
Lymphocytes
Phytohemagglutinins
Interleukin-2 Receptors
Messenger RNA
Neurotransmitter Agents
T-Cell Antigen Receptor
Reverse Transcriptase Polymerase Chain Reaction
Northern Blotting
Nervous System
oxotremorine M
Immune System
Up-Regulation
Fluorescence
Enzyme-Linked Immunosorbent Assay
Cytokines
T-Lymphocytes
DNA

ASJC Scopus subject areas

  • Pharmacology

Cite this

Stimulatory roles of muscarinic acetylcholine receptors on T cell antigen receptor/CD3 complex-mediated interleukin-2 production in human peripheral blood lymphocytes. / Fujino, Hiromichi; Kitamura, Yoshihisa; Yada, Takeshi; Uehara, Takashi; Nomura, Yasuyuki.

In: Molecular Pharmacology, Vol. 51, No. 6, 06.1997, p. 1007-1014.

Research output: Contribution to journalArticle

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