TY - JOUR
T1 - Stimulatory effects of 4-methylcatechol, dopamine and levodopa on the expression of metallothionein-III (GIF) mRNA in immortalized mouse brain glial cells (VR-2g)
AU - Sogawa, Chiharu
AU - Nakanishi, Tohru
AU - Sogawa, Norio
AU - Ishii, Kazuhiro
AU - Ogawa, Norio
AU - Takigawa, Masaharu
AU - Furuta, Hiroaki
N1 - Funding Information:
This study was supported in part by Grants-in-Aid for Scientific Research from the Japanese Ministry of Welfare, and from the Japanese Ministry of Education, Science, Sports and Culture.
PY - 1998/5/11
Y1 - 1998/5/11
N2 - Metallothionein (MT)-III, originally discovered as a growth inhibitory factor (GIF), is a brain specific isomer of MTs and is markedly reduced in the brain of Alzheimer's disease patients (AD) and in several other neurodegenerative diseases. We analyzed the level and regulation of mRNA expression of MT-III in immortalized fetal mouse brain glial cells (VR-2g) by reverse transcriptase-polymerase chain reaction (RT-PCR). The basal expression level of MT-III mRNA is very low in VR-2g cells. 4-Methylcatechol, dopamine (DA) and levodopa (L-3,4-dihydroxyphenylalanine), which stimulate the synthesis of nerve growth factor (NGF), further increased the expression of MT-III mRNA in VR-2g cells.
AB - Metallothionein (MT)-III, originally discovered as a growth inhibitory factor (GIF), is a brain specific isomer of MTs and is markedly reduced in the brain of Alzheimer's disease patients (AD) and in several other neurodegenerative diseases. We analyzed the level and regulation of mRNA expression of MT-III in immortalized fetal mouse brain glial cells (VR-2g) by reverse transcriptase-polymerase chain reaction (RT-PCR). The basal expression level of MT-III mRNA is very low in VR-2g cells. 4-Methylcatechol, dopamine (DA) and levodopa (L-3,4-dihydroxyphenylalanine), which stimulate the synthesis of nerve growth factor (NGF), further increased the expression of MT-III mRNA in VR-2g cells.
KW - 4- Methylcatechol
KW - Dopamine
KW - Glial cell
KW - Growth inhibitory factor
KW - Levodopa
KW - Metallothionein-III
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U2 - 10.1016/S0006-8993(98)00239-X
DO - 10.1016/S0006-8993(98)00239-X
M3 - Article
C2 - 9593981
AN - SCOPUS:0032507694
SN - 0006-8993
VL - 792
SP - 335
EP - 339
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 2
ER -