Stimulatory effects of 4-methylcatechol, dopamine and levodopa on the expression of metallothionein-III (GIF) mRNA in immortalized mouse brain glial cells (VR-2g)

Chiharu Sogawa, Tohru Nakanishi, Norio Sogawa, Kazuhiro Ishii, Norio Ogawa, Masaharu Takigawa, Hiroaki Furuta

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Metallothionein (MT)-III, originally discovered as a growth inhibitory factor (GIF), is a brain specific isomer of MTs and is markedly reduced in the brain of Alzheimer's disease patients (AD) and in several other neurodegenerative diseases. We analyzed the level and regulation of mRNA expression of MT-III in immortalized fetal mouse brain glial cells (VR-2g) by reverse transcriptase-polymerase chain reaction (RT-PCR). The basal expression level of MT-III mRNA is very low in VR-2g cells. 4-Methylcatechol, dopamine (DA) and levodopa (L-3,4-dihydroxyphenylalanine), which stimulate the synthesis of nerve growth factor (NGF), further increased the expression of MT-III mRNA in VR-2g cells.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalBrain Research
Volume792
Issue number2
DOIs
Publication statusPublished - May 11 1998

Fingerprint

Levodopa
Neuroglia
Dopamine
Messenger RNA
Brain
Brain Diseases
Nerve Growth Factor
Reverse Transcriptase Polymerase Chain Reaction
Neurodegenerative Diseases
Alzheimer Disease
growth inhibitory factor
4-methylcatechol

Keywords

  • 4- Methylcatechol
  • Dopamine
  • Glial cell
  • Growth inhibitory factor
  • Levodopa
  • Metallothionein-III

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Stimulatory effects of 4-methylcatechol, dopamine and levodopa on the expression of metallothionein-III (GIF) mRNA in immortalized mouse brain glial cells (VR-2g). / Sogawa, Chiharu; Nakanishi, Tohru; Sogawa, Norio; Ishii, Kazuhiro; Ogawa, Norio; Takigawa, Masaharu; Furuta, Hiroaki.

In: Brain Research, Vol. 792, No. 2, 11.05.1998, p. 335-339.

Research output: Contribution to journalArticle

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AU - Sogawa, Norio

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AU - Furuta, Hiroaki

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