Stimulation of plasma membrane h+-transport activity in barley roots by salt stress

Minobu Kasai, Kousei Yamashita, Minobu Kasai, Yoko Yamamoto, Hideaki Matsumoto

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)

    Abstract

    Plasma membrane vesicles were prepared from barley roots (Hordeum vulgare cv. Kikaihadaka) using the phase partitioning method. H+-transport activity could be measured without membrane inversion treatment, since the plasma membrane vesicles showed a latency value of approximately 50% for the activity. Following the exposure to NaCl, alterations of the plasma membrane H+-ATPase and H+-transport activity were investigated. After barley roots were treated with 200 mol m−3NaCl for 1 d, both plasma membrane H+-ATPase activity and H+-transport activity assayed in the presence of nitrate were reduced as compared with those of control roots. However, in the salinized roots, the H+ -transport activity measured with chloride was higher than that of control roots, though the H+-ATPase activity was lower. These results suggest that the permeability of the plasma membrane to chloride may increase by salt stress.

    Original languageEnglish
    Pages (from-to)555-563
    Number of pages9
    JournalSoil Science and Plant Nutrition
    Volume40
    Issue number4
    DOIs
    Publication statusPublished - Dec 1994

    Keywords

    • -ATPase
    • Barley roots
    • Chloride
    • H-transport
    • Plasma membrane

    ASJC Scopus subject areas

    • Soil Science
    • Plant Science

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