Stimulation of plasma membrane h+-transport activity in barley roots by salt stress

Minobu Kasai, Kousei Yamashita, Minobu Kasai, Yoko Yamamoto, Hideaki Matsumoto

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19 Citations (Scopus)


Plasma membrane vesicles were prepared from barley roots (Hordeum vulgare cv. Kikaihadaka) using the phase partitioning method. H+-transport activity could be measured without membrane inversion treatment, since the plasma membrane vesicles showed a latency value of approximately 50% for the activity. Following the exposure to NaCl, alterations of the plasma membrane H+-ATPase and H+-transport activity were investigated. After barley roots were treated with 200 mol m−3NaCl for 1 d, both plasma membrane H+-ATPase activity and H+-transport activity assayed in the presence of nitrate were reduced as compared with those of control roots. However, in the salinized roots, the H+ -transport activity measured with chloride was higher than that of control roots, though the H+-ATPase activity was lower. These results suggest that the permeability of the plasma membrane to chloride may increase by salt stress.

Original languageEnglish
Pages (from-to)555-563
Number of pages9
JournalSoil Science and Plant Nutrition
Issue number4
Publication statusPublished - Dec 1994


  • -ATPase
  • Barley roots
  • Chloride
  • H-transport
  • Plasma membrane

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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