Stimulation of H+extrusion and plasma membrane H+-ATPase activity of barley roots by ammonium-treatment

Kousei Yamashita, Bunich Ezaki, Mineo Shibasaka, Yoko Yamamoto, Hideo Sasakawa, Minobu Kasai, Hideaki Matsumoto

Research output: Contribution to journal › Article

18 Citations (Scopus)

Abstract

H+extrusion and the plasma membrane H+-ATPase activity were analyzed using barley roots exposed to ammonium. H+extrusion from roots was enhanced 4 h after the addition of 5 mol m-3ammonium to the medium. However, the stimulation of the plasma membrane H+-ATPase activity occurred at least 8 h after the addition of ammonium. Furthermore, this stimulation was found to be caused by the increase in the content of H+-ATPase proteins based on the results of immunoassay. These results indicate that H+was actively extruded to the medium through the increase in the amount of plasma membrane H+-ATPase proteins, when barley roots were exposed to ammonium for more than 8 h. Thus, it is assumed that in the long term exposure to ammonium nitrogen, ammonium-induced stimulation of the H+-ATPase activity may be important to maintain intracellular pH in root cells under the acidic conditions associated with the uptake of ammonium.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalSoil Science and Plant Nutrition
Volume41
Issue number1
DOIs
Publication statusPublished - 1995

Fingerprint

H-transporting ATP synthase
extrusion
barley
plasma membrane
ammonium
membrane
plasma
barley protein
ammonium nitrogen
chronic exposure
immunoassays
protein
immunoassay
uptake mechanisms
nitrogen
proteins
cells

Keywords

  • Ammonium
  • Barley roots
  • Hextrusion
  • Plasma membrane
  • Stimulation of H-ATPase

ASJC Scopus subject areas

  • Soil Science
  • Plant Science
  • Agronomy and Crop Science

Cite this

Yamashita, K., Ezaki, B., Shibasaka, M., Yamamoto, Y., Sasakawa, H., Kasai, M., & Matsumoto, H. (1995). Stimulation of H+extrusion and plasma membrane H+-ATPase activity of barley roots by ammonium-treatment. Soil Science and Plant Nutrition, 41(1), 133-140. https://doi.org/10.1080/00380768.1995.10419566

Stimulation of H+extrusion and plasma membrane H+-ATPase activity of barley roots by ammonium-treatment. / Yamashita, Kousei; Ezaki, Bunich; Shibasaka, Mineo; Yamamoto, Yoko; Sasakawa, Hideo; Kasai, Minobu; Matsumoto, Hideaki.

In: Soil Science and Plant Nutrition, Vol. 41, No. 1, 1995, p. 133-140.

Research output: Contribution to journal › Article

Yamashita, K, Ezaki, B, Shibasaka, M, Yamamoto, Y, Sasakawa, H, Kasai, M & Matsumoto, H 1995, 'Stimulation of H+extrusion and plasma membrane H+-ATPase activity of barley roots by ammonium-treatment', Soil Science and Plant Nutrition, vol. 41, no. 1, pp. 133-140. https://doi.org/10.1080/00380768.1995.10419566
Yamashita K, Ezaki B, Shibasaka M, Yamamoto Y, Sasakawa H, Kasai M et al. Stimulation of H+extrusion and plasma membrane H+-ATPase activity of barley roots by ammonium-treatment. Soil Science and Plant Nutrition. 1995;41(1):133-140. https://doi.org/10.1080/00380768.1995.10419566
Yamashita, Kousei ; Ezaki, Bunich ; Shibasaka, Mineo ; Yamamoto, Yoko ; Sasakawa, Hideo ; Kasai, Minobu ; Matsumoto, Hideaki. / Stimulation of H+extrusion and plasma membrane H+-ATPase activity of barley roots by ammonium-treatment. In: Soil Science and Plant Nutrition. 1995 ; Vol. 41, No. 1. pp. 133-140.
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AB - H+extrusion and the plasma membrane H+-ATPase activity were analyzed using barley roots exposed to ammonium. H+extrusion from roots was enhanced 4 h after the addition of 5 mol m-3ammonium to the medium. However, the stimulation of the plasma membrane H+-ATPase activity occurred at least 8 h after the addition of ammonium. Furthermore, this stimulation was found to be caused by the increase in the content of H+-ATPase proteins based on the results of immunoassay. These results indicate that H+was actively extruded to the medium through the increase in the amount of plasma membrane H+-ATPase proteins, when barley roots were exposed to ammonium for more than 8 h. Thus, it is assumed that in the long term exposure to ammonium nitrogen, ammonium-induced stimulation of the H+-ATPase activity may be important to maintain intracellular pH in root cells under the acidic conditions associated with the uptake of ammonium.

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