Stimulation of glycolysis in anaerobic elongation of pondweed (Potamogeton distinctus) turions

Tatsuhisa Sato, Taro Harada, Kimiharu Ishizawa

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Stem segments prepared from pondweed (Potamogeton distinctus A. Benn.) turions (overwintering buds) elongate in anaerobic conditions, whereas there is almost no elongation in air. The anaerobic elongation was accompanied by a decrease in dry weights of stem segments, mainly due to consumption of storage starch in the amyloplasts of stem cells. On the other hand, total contents of amino acids increased in stem segments, in which contents of alanine, valine, leucine, and isoleucine increased, but contents of asparatic acid decreased. Moreover, contents of lactate in stem tissues increased at an early stage of anaerobic incubation. In tracer experiments with 14C-glucose, 14C incorporation into stem tissues in anoxia was only half of that in normoxia. However, conversion of 14C to ethanol occurred exclusively in anoxia. 14C-labelled metabolites were analysed by two-dimensional cellulose thin-layer chromatography. 14C incorporation into sucrose and alanine was significantly increased in anoxia. The activity of alanine aminotransferase was enhanced by anoxia, suggesting that pyruvate is a precursor of alanine synthesis. The results suggest that pond-weed turions produce energy necessary for anaerobic elongation by activating conversion of storage starch in the amyloplasts to ethanol, alanine and lactate.

Original languageEnglish
Pages (from-to)1847-1856
Number of pages10
JournalJournal of Experimental Botany
Volume53
Issue number376
Publication statusPublished - Sep 2002
Externally publishedYes

Fingerprint

Potamogetonaceae
glycolysis
Glycolysis
Alanine
alanine
hypoxia
Plastids
stems
amyloplasts
Starch
Lactic Acid
Ethanol
lactates
ethanol
Isoleucine
starch
Valine
Thin Layer Chromatography
Alanine Transaminase
Pyruvic Acid

Keywords

  • Alanine
  • Amyloplast
  • Anoxia
  • Elongation
  • Ethanol
  • Fermentation
  • Glycolysis
  • Lactate
  • Potamogeton distinctus A. Benn.
  • Turion

ASJC Scopus subject areas

  • Plant Science

Cite this

Stimulation of glycolysis in anaerobic elongation of pondweed (Potamogeton distinctus) turions. / Sato, Tatsuhisa; Harada, Taro; Ishizawa, Kimiharu.

In: Journal of Experimental Botany, Vol. 53, No. 376, 09.2002, p. 1847-1856.

Research output: Contribution to journalArticle

@article{64ff7a4e792d4201beab791222371b65,
title = "Stimulation of glycolysis in anaerobic elongation of pondweed (Potamogeton distinctus) turions",
abstract = "Stem segments prepared from pondweed (Potamogeton distinctus A. Benn.) turions (overwintering buds) elongate in anaerobic conditions, whereas there is almost no elongation in air. The anaerobic elongation was accompanied by a decrease in dry weights of stem segments, mainly due to consumption of storage starch in the amyloplasts of stem cells. On the other hand, total contents of amino acids increased in stem segments, in which contents of alanine, valine, leucine, and isoleucine increased, but contents of asparatic acid decreased. Moreover, contents of lactate in stem tissues increased at an early stage of anaerobic incubation. In tracer experiments with 14C-glucose, 14C incorporation into stem tissues in anoxia was only half of that in normoxia. However, conversion of 14C to ethanol occurred exclusively in anoxia. 14C-labelled metabolites were analysed by two-dimensional cellulose thin-layer chromatography. 14C incorporation into sucrose and alanine was significantly increased in anoxia. The activity of alanine aminotransferase was enhanced by anoxia, suggesting that pyruvate is a precursor of alanine synthesis. The results suggest that pond-weed turions produce energy necessary for anaerobic elongation by activating conversion of storage starch in the amyloplasts to ethanol, alanine and lactate.",
keywords = "Alanine, Amyloplast, Anoxia, Elongation, Ethanol, Fermentation, Glycolysis, Lactate, Potamogeton distinctus A. Benn., Turion",
author = "Tatsuhisa Sato and Taro Harada and Kimiharu Ishizawa",
year = "2002",
month = "9",
language = "English",
volume = "53",
pages = "1847--1856",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "376",

}

TY - JOUR

T1 - Stimulation of glycolysis in anaerobic elongation of pondweed (Potamogeton distinctus) turions

AU - Sato, Tatsuhisa

AU - Harada, Taro

AU - Ishizawa, Kimiharu

PY - 2002/9

Y1 - 2002/9

N2 - Stem segments prepared from pondweed (Potamogeton distinctus A. Benn.) turions (overwintering buds) elongate in anaerobic conditions, whereas there is almost no elongation in air. The anaerobic elongation was accompanied by a decrease in dry weights of stem segments, mainly due to consumption of storage starch in the amyloplasts of stem cells. On the other hand, total contents of amino acids increased in stem segments, in which contents of alanine, valine, leucine, and isoleucine increased, but contents of asparatic acid decreased. Moreover, contents of lactate in stem tissues increased at an early stage of anaerobic incubation. In tracer experiments with 14C-glucose, 14C incorporation into stem tissues in anoxia was only half of that in normoxia. However, conversion of 14C to ethanol occurred exclusively in anoxia. 14C-labelled metabolites were analysed by two-dimensional cellulose thin-layer chromatography. 14C incorporation into sucrose and alanine was significantly increased in anoxia. The activity of alanine aminotransferase was enhanced by anoxia, suggesting that pyruvate is a precursor of alanine synthesis. The results suggest that pond-weed turions produce energy necessary for anaerobic elongation by activating conversion of storage starch in the amyloplasts to ethanol, alanine and lactate.

AB - Stem segments prepared from pondweed (Potamogeton distinctus A. Benn.) turions (overwintering buds) elongate in anaerobic conditions, whereas there is almost no elongation in air. The anaerobic elongation was accompanied by a decrease in dry weights of stem segments, mainly due to consumption of storage starch in the amyloplasts of stem cells. On the other hand, total contents of amino acids increased in stem segments, in which contents of alanine, valine, leucine, and isoleucine increased, but contents of asparatic acid decreased. Moreover, contents of lactate in stem tissues increased at an early stage of anaerobic incubation. In tracer experiments with 14C-glucose, 14C incorporation into stem tissues in anoxia was only half of that in normoxia. However, conversion of 14C to ethanol occurred exclusively in anoxia. 14C-labelled metabolites were analysed by two-dimensional cellulose thin-layer chromatography. 14C incorporation into sucrose and alanine was significantly increased in anoxia. The activity of alanine aminotransferase was enhanced by anoxia, suggesting that pyruvate is a precursor of alanine synthesis. The results suggest that pond-weed turions produce energy necessary for anaerobic elongation by activating conversion of storage starch in the amyloplasts to ethanol, alanine and lactate.

KW - Alanine

KW - Amyloplast

KW - Anoxia

KW - Elongation

KW - Ethanol

KW - Fermentation

KW - Glycolysis

KW - Lactate

KW - Potamogeton distinctus A. Benn.

KW - Turion

UR - http://www.scopus.com/inward/record.url?scp=0036707545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036707545&partnerID=8YFLogxK

M3 - Article

VL - 53

SP - 1847

EP - 1856

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 376

ER -