Steroids mediate resistance to the bactericidal effect of phosphatidylcholines against Helicobacter pylori

Hirofumi Shimomura, Kouichi Hosoda, Shunji Hayashi, Kenji Yokota, Keiji Oguma, Yoshikazu Hirai

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Helicobacter pylori assimilates various steroids as membrane lipid components, but it can also survive in the absence of steroids. It thus remains to be clarified as to why the organism relies on steroid physiologically. In this study, we have found that phosphatidylcholine carrying a linoleic acid molecule or arachidonic acid molecule has the potential to kill steroid-free H. pylori. The bactericidal action of phosphatidylcholines against H. pylori was due to the lytic activity of the phosphatidylcholines themselves and not due to the lytic activity of the unsaturated fatty acids or lyso-phosphatidylcholine resulting from the hydrolysis of the phosphatidylcholines. In contrast to the steroid-free H. pylori, the organism that absorbed and glucosylated free cholesterol was unaffected by the bactericidal action of the phosphatidylcholines. Similarly, H. pylori that absorbed estrone without glucosylating it also resisted the bactericidal action of the phosphatidylcholines. The steroids absorbed by H. pylori existed in both the outer and inner membranes, while the glucosyl-steroids produced via the steroid absorption were localized in the outer membrane rather than in the inner membrane. These results indicate that H. pylori absorbs the steroids to reinforce the membrane lipid barrier and thereby expresses resistance to the bacteriolytic action of hydrophobic compounds such as phosphatidylcholine.

Original languageEnglish
Pages (from-to)84-94
Number of pages11
JournalFEMS Microbiology Letters
Volume301
Issue number1
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Phosphatidylcholines
Helicobacter pylori
Steroids
Membrane Lipids
Membranes
Estrone
Linoleic Acid
Unsaturated Fatty Acids
Arachidonic Acid
Hydrolysis
Cholesterol

Keywords

  • Cholesterol
  • Estrone
  • Helicobacter pylori
  • Phosphatidylcholine
  • Testosterone

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Steroids mediate resistance to the bactericidal effect of phosphatidylcholines against Helicobacter pylori. / Shimomura, Hirofumi; Hosoda, Kouichi; Hayashi, Shunji; Yokota, Kenji; Oguma, Keiji; Hirai, Yoshikazu.

In: FEMS Microbiology Letters, Vol. 301, No. 1, 01.2009, p. 84-94.

Research output: Contribution to journalArticle

Shimomura, Hirofumi ; Hosoda, Kouichi ; Hayashi, Shunji ; Yokota, Kenji ; Oguma, Keiji ; Hirai, Yoshikazu. / Steroids mediate resistance to the bactericidal effect of phosphatidylcholines against Helicobacter pylori. In: FEMS Microbiology Letters. 2009 ; Vol. 301, No. 1. pp. 84-94.
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