Stepwise changes in viable but nonculturable Vibrio cholerae cells

Daisuke Imamura, Tamaki Mizuno, Shin Ichi Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.

Original languageEnglish
Pages (from-to)305-310
Number of pages6
JournalMICROBIOLOGY and IMMUNOLOGY
Volume59
Issue number5
DOIs
Publication statusPublished - May 1 2015

Keywords

  • Resuscitation
  • Viable but nonculturable
  • Vibrio cholerae

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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