Stepwise changes in viable but nonculturable Vibrio cholerae cells

Daisuke Imamura, Tamaki Mizuno, Shin-ichi Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.

Original languageEnglish
Pages (from-to)305-310
Number of pages6
JournalMicrobiology and Immunology
Volume59
Issue number5
DOIs
Publication statusPublished - May 1 2015

Fingerprint

Vibrio cholerae
Vibrio cholerae O139
Vibrio cholerae O1
HT29 Cells
Cell Extracts
Fluorescence Microscopy
Resuscitation
Catalase
Colon
Adenocarcinoma
Growth

Keywords

  • Resuscitation
  • Viable but nonculturable
  • Vibrio cholerae

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology

Cite this

Stepwise changes in viable but nonculturable Vibrio cholerae cells. / Imamura, Daisuke; Mizuno, Tamaki; Miyoshi, Shin-ichi; Shinoda, Sumio.

In: Microbiology and Immunology, Vol. 59, No. 5, 01.05.2015, p. 305-310.

Research output: Contribution to journalArticle

@article{e966cf661d434fcfa6c86330f7fafef9,
title = "Stepwise changes in viable but nonculturable Vibrio cholerae cells",
abstract = "Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.",
keywords = "Resuscitation, Viable but nonculturable, Vibrio cholerae",
author = "Daisuke Imamura and Tamaki Mizuno and Shin-ichi Miyoshi and Sumio Shinoda",
year = "2015",
month = "5",
day = "1",
doi = "10.1111/1348-0421.12246",
language = "English",
volume = "59",
pages = "305--310",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "5",

}

TY - JOUR

T1 - Stepwise changes in viable but nonculturable Vibrio cholerae cells

AU - Imamura, Daisuke

AU - Mizuno, Tamaki

AU - Miyoshi, Shin-ichi

AU - Shinoda, Sumio

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.

AB - Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.

KW - Resuscitation

KW - Viable but nonculturable

KW - Vibrio cholerae

UR - http://www.scopus.com/inward/record.url?scp=84929725235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929725235&partnerID=8YFLogxK

U2 - 10.1111/1348-0421.12246

DO - 10.1111/1348-0421.12246

M3 - Article

C2 - 25664673

AN - SCOPUS:84929725235

VL - 59

SP - 305

EP - 310

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 5

ER -