Stepwise changes in viable but nonculturable Vibrio cholerae cells

Daisuke Imamura, Tamaki Mizuno, Shin Ichi Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Many bacterial species are known to become viable but nonculturable (VBNC) under conditions that are unsuitable for growth. In this study, the requirements for resuscitation of VBNC-state Vibrio cholerae cells were found to change over time. Although VBNC cells could initially be converted to culturable by treatment with catalase or HT-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. However, fluorescence microscopy revealed the presence of live cells in this state, from which VBNC cells were resuscitated by co-cultivation with HT-29 human colon adenocarcinoma cells. Ultimately, all cells entered a state from which they could not be resuscitated, even by co-cultivation with HT-29. These characteristic changes in VBNC-state cells were a common feature of strains in both V. cholerae O1 and O139 serogroups. Thus, the VBNC state of V. cholerae is not a single property but continues to change over time.

Original languageEnglish
Pages (from-to)305-310
Number of pages6
JournalMICROBIOLOGY and IMMUNOLOGY
Volume59
Issue number5
DOIs
Publication statusPublished - May 1 2015

Keywords

  • Resuscitation
  • Viable but nonculturable
  • Vibrio cholerae

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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