Stearylamine Changes the Liposomal Shape from MLVs to LUVs

Shoko Yokoyama, Akiko Inagaki, Koji Tsuchiya, Hideki Sakai, Tomohiro Imura, Takahiro Ohkubo, Nobuyuki Tsubaki, Masahiko Abe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The shape of mixed dipalmitoylphosphatidylcholine (DPPC)/stearylamine (SA) liposomes prepared by the Bangham method was examined using freeze-fracture electron microscopy. The shape of the mixed DPPC/SA liposomes (XSA = 0.05) was not that of multilamellar vesicles (MLVs) but large unilamellar vesicles (LUVs) with a diameter of approximately 1.0 μm. These facts overthrow the preconceived idea that the shape of the DPPC/SA liposomes prepared by the Bangham method is that of MLVs. The trapping efficiency of the mixed DPPC/SA liposomes (XSA = 0.05) was ten times greater than that of the DPPC liposomes, since the DPPC liposomes were MLVs. The data of the DSC peak for the DPPC/SA liposomes also indicated that the shape of the DPPC/SA liposomes is that of LUVs. We emphasize that the shape of the DPPC liposomes changes from that of MLVs to LUVs by the mixing of a small amount of SA into the DPPC liposomes.

Original languageEnglish
Pages (from-to)251-254
Number of pages4
JournalJournal of Oleo Science
Volume54
Issue number4
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

1,2-Dipalmitoylphosphatidylcholine
Unilamellar Liposomes
Liposomes
stearylamine
Electron microscopy

Keywords

  • freeze-fracture electron microscopy
  • large unilamellar vesicle
  • mixed liposome
  • stearylamine
  • trapping efficiency

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Yokoyama, S., Inagaki, A., Tsuchiya, K., Sakai, H., Imura, T., Ohkubo, T., ... Abe, M. (2005). Stearylamine Changes the Liposomal Shape from MLVs to LUVs. Journal of Oleo Science, 54(4), 251-254. https://doi.org/10.5650/jos.54.251

Stearylamine Changes the Liposomal Shape from MLVs to LUVs. / Yokoyama, Shoko; Inagaki, Akiko; Tsuchiya, Koji; Sakai, Hideki; Imura, Tomohiro; Ohkubo, Takahiro; Tsubaki, Nobuyuki; Abe, Masahiko.

In: Journal of Oleo Science, Vol. 54, No. 4, 2005, p. 251-254.

Research output: Contribution to journalArticle

Yokoyama, S, Inagaki, A, Tsuchiya, K, Sakai, H, Imura, T, Ohkubo, T, Tsubaki, N & Abe, M 2005, 'Stearylamine Changes the Liposomal Shape from MLVs to LUVs', Journal of Oleo Science, vol. 54, no. 4, pp. 251-254. https://doi.org/10.5650/jos.54.251
Yokoyama, Shoko ; Inagaki, Akiko ; Tsuchiya, Koji ; Sakai, Hideki ; Imura, Tomohiro ; Ohkubo, Takahiro ; Tsubaki, Nobuyuki ; Abe, Masahiko. / Stearylamine Changes the Liposomal Shape from MLVs to LUVs. In: Journal of Oleo Science. 2005 ; Vol. 54, No. 4. pp. 251-254.
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