Abstract
The shape of mixed dipalmitoylphosphatidylcholine (DPPC)/stearylamine (SA) liposomes prepared by the Bangham method was examined using freeze-fracture electron microscopy. The shape of the mixed DPPC/SA liposomes (XSA = 0.05) was not that of multilamellar vesicles (MLVs) but large unilamellar vesicles (LUVs) with a diameter of approximately 1.0 μm. These facts overthrow the preconceived idea that the shape of the DPPC/SA liposomes prepared by the Bangham method is that of MLVs. The trapping efficiency of the mixed DPPC/SA liposomes (XSA = 0.05) was ten times greater than that of the DPPC liposomes, since the DPPC liposomes were MLVs. The data of the DSC peak for the DPPC/SA liposomes also indicated that the shape of the DPPC/SA liposomes is that of LUVs. We emphasize that the shape of the DPPC liposomes changes from that of MLVs to LUVs by the mixing of a small amount of SA into the DPPC liposomes.
| Original language | English |
|---|---|
| Pages (from-to) | 251-254 |
| Number of pages | 4 |
| Journal | Journal of Oleo Science |
| Volume | 54 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2005 |
| Externally published | Yes |
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Keywords
- freeze-fracture electron microscopy
- large unilamellar vesicle
- mixed liposome
- stearylamine
- trapping efficiency
ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)
Cite this
Stearylamine Changes the Liposomal Shape from MLVs to LUVs. / Yokoyama, Shoko; Inagaki, Akiko; Tsuchiya, Koji; Sakai, Hideki; Imura, Tomohiro; Ohkubo, Takahiro; Tsubaki, Nobuyuki; Abe, Masahiko.
In: Journal of Oleo Science, Vol. 54, No. 4, 2005, p. 251-254.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stearylamine Changes the Liposomal Shape from MLVs to LUVs
AU - Yokoyama, Shoko
AU - Inagaki, Akiko
AU - Tsuchiya, Koji
AU - Sakai, Hideki
AU - Imura, Tomohiro
AU - Ohkubo, Takahiro
AU - Tsubaki, Nobuyuki
AU - Abe, Masahiko
PY - 2005
Y1 - 2005
N2 - The shape of mixed dipalmitoylphosphatidylcholine (DPPC)/stearylamine (SA) liposomes prepared by the Bangham method was examined using freeze-fracture electron microscopy. The shape of the mixed DPPC/SA liposomes (XSA = 0.05) was not that of multilamellar vesicles (MLVs) but large unilamellar vesicles (LUVs) with a diameter of approximately 1.0 μm. These facts overthrow the preconceived idea that the shape of the DPPC/SA liposomes prepared by the Bangham method is that of MLVs. The trapping efficiency of the mixed DPPC/SA liposomes (XSA = 0.05) was ten times greater than that of the DPPC liposomes, since the DPPC liposomes were MLVs. The data of the DSC peak for the DPPC/SA liposomes also indicated that the shape of the DPPC/SA liposomes is that of LUVs. We emphasize that the shape of the DPPC liposomes changes from that of MLVs to LUVs by the mixing of a small amount of SA into the DPPC liposomes.
AB - The shape of mixed dipalmitoylphosphatidylcholine (DPPC)/stearylamine (SA) liposomes prepared by the Bangham method was examined using freeze-fracture electron microscopy. The shape of the mixed DPPC/SA liposomes (XSA = 0.05) was not that of multilamellar vesicles (MLVs) but large unilamellar vesicles (LUVs) with a diameter of approximately 1.0 μm. These facts overthrow the preconceived idea that the shape of the DPPC/SA liposomes prepared by the Bangham method is that of MLVs. The trapping efficiency of the mixed DPPC/SA liposomes (XSA = 0.05) was ten times greater than that of the DPPC liposomes, since the DPPC liposomes were MLVs. The data of the DSC peak for the DPPC/SA liposomes also indicated that the shape of the DPPC/SA liposomes is that of LUVs. We emphasize that the shape of the DPPC liposomes changes from that of MLVs to LUVs by the mixing of a small amount of SA into the DPPC liposomes.
KW - freeze-fracture electron microscopy
KW - large unilamellar vesicle
KW - mixed liposome
KW - stearylamine
KW - trapping efficiency
UR - http://www.scopus.com/inward/record.url?scp=85015684874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85015684874&partnerID=8YFLogxK
U2 - 10.5650/jos.54.251
DO - 10.5650/jos.54.251
M3 - Article
VL - 54
SP - 251
EP - 254
JO - Journal of Oleo Science
JF - Journal of Oleo Science
SN - 1345-8957
IS - 4
ER -