Stearylamine Changes the Liposomal Shape from MLVs to LUVs

Shoko Yokoyama; Akiko Inagaki; Koji Tsuchiya; Hideki Sakai; Tomohiro Imura; Takahiro Ohkubo; Nobuyuki Tsubaki; Masahiko Abe

doi:10.5650/jos.54.251

Stearylamine Changes the Liposomal Shape from MLVs to LUVs

Shoko Yokoyama, Akiko Inagaki, Koji Tsuchiya, Hideki Sakai, Tomohiro Imura, Takahiro Ohkubo, Nobuyuki Tsubaki, Masahiko Abe

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The shape of mixed dipalmitoylphosphatidylcholine (DPPC)/stearylamine (SA) liposomes prepared by the Bangham method was examined using freeze-fracture electron microscopy. The shape of the mixed DPPC/SA liposomes (X_SA = 0.05) was not that of multilamellar vesicles (MLVs) but large unilamellar vesicles (LUVs) with a diameter of approximately 1.0 μm. These facts overthrow the preconceived idea that the shape of the DPPC/SA liposomes prepared by the Bangham method is that of MLVs. The trapping efficiency of the mixed DPPC/SA liposomes (X_SA = 0.05) was ten times greater than that of the DPPC liposomes, since the DPPC liposomes were MLVs. The data of the DSC peak for the DPPC/SA liposomes also indicated that the shape of the DPPC/SA liposomes is that of LUVs. We emphasize that the shape of the DPPC liposomes changes from that of MLVs to LUVs by the mixing of a small amount of SA into the DPPC liposomes.

Original language	English
Pages (from-to)	251-254
Number of pages	4
Journal	Journal of Oleo Science
Volume	54
Issue number	4
DOIs	https://doi.org/10.5650/jos.54.251
Publication status	Published - 2005
Externally published	Yes

Keywords

freeze-fracture electron microscopy
large unilamellar vesicle
mixed liposome
stearylamine
trapping efficiency

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

Access to Document

10.5650/jos.54.251

Cite this

@article{b5615f2a721243938af0e874cc6f9181,

title = "Stearylamine Changes the Liposomal Shape from MLVs to LUVs",

abstract = "The shape of mixed dipalmitoylphosphatidylcholine (DPPC)/stearylamine (SA) liposomes prepared by the Bangham method was examined using freeze-fracture electron microscopy. The shape of the mixed DPPC/SA liposomes (XSA = 0.05) was not that of multilamellar vesicles (MLVs) but large unilamellar vesicles (LUVs) with a diameter of approximately 1.0 μm. These facts overthrow the preconceived idea that the shape of the DPPC/SA liposomes prepared by the Bangham method is that of MLVs. The trapping efficiency of the mixed DPPC/SA liposomes (XSA = 0.05) was ten times greater than that of the DPPC liposomes, since the DPPC liposomes were MLVs. The data of the DSC peak for the DPPC/SA liposomes also indicated that the shape of the DPPC/SA liposomes is that of LUVs. We emphasize that the shape of the DPPC liposomes changes from that of MLVs to LUVs by the mixing of a small amount of SA into the DPPC liposomes.",

keywords = "freeze-fracture electron microscopy, large unilamellar vesicle, mixed liposome, stearylamine, trapping efficiency",

author = "Shoko Yokoyama and Akiko Inagaki and Koji Tsuchiya and Hideki Sakai and Tomohiro Imura and Takahiro Ohkubo and Nobuyuki Tsubaki and Masahiko Abe",

year = "2005",

doi = "10.5650/jos.54.251",

language = "English",

volume = "54",

pages = "251--254",

journal = "Journal of Oleo Science",

issn = "1345-8957",

publisher = "Japan Oil Chemists Society",

number = "4",

}

TY - JOUR

T1 - Stearylamine Changes the Liposomal Shape from MLVs to LUVs

AU - Yokoyama, Shoko

AU - Inagaki, Akiko

AU - Tsuchiya, Koji

AU - Sakai, Hideki

AU - Imura, Tomohiro

AU - Ohkubo, Takahiro

AU - Tsubaki, Nobuyuki

AU - Abe, Masahiko

PY - 2005

Y1 - 2005

N2 - The shape of mixed dipalmitoylphosphatidylcholine (DPPC)/stearylamine (SA) liposomes prepared by the Bangham method was examined using freeze-fracture electron microscopy. The shape of the mixed DPPC/SA liposomes (XSA = 0.05) was not that of multilamellar vesicles (MLVs) but large unilamellar vesicles (LUVs) with a diameter of approximately 1.0 μm. These facts overthrow the preconceived idea that the shape of the DPPC/SA liposomes prepared by the Bangham method is that of MLVs. The trapping efficiency of the mixed DPPC/SA liposomes (XSA = 0.05) was ten times greater than that of the DPPC liposomes, since the DPPC liposomes were MLVs. The data of the DSC peak for the DPPC/SA liposomes also indicated that the shape of the DPPC/SA liposomes is that of LUVs. We emphasize that the shape of the DPPC liposomes changes from that of MLVs to LUVs by the mixing of a small amount of SA into the DPPC liposomes.

AB - The shape of mixed dipalmitoylphosphatidylcholine (DPPC)/stearylamine (SA) liposomes prepared by the Bangham method was examined using freeze-fracture electron microscopy. The shape of the mixed DPPC/SA liposomes (XSA = 0.05) was not that of multilamellar vesicles (MLVs) but large unilamellar vesicles (LUVs) with a diameter of approximately 1.0 μm. These facts overthrow the preconceived idea that the shape of the DPPC/SA liposomes prepared by the Bangham method is that of MLVs. The trapping efficiency of the mixed DPPC/SA liposomes (XSA = 0.05) was ten times greater than that of the DPPC liposomes, since the DPPC liposomes were MLVs. The data of the DSC peak for the DPPC/SA liposomes also indicated that the shape of the DPPC/SA liposomes is that of LUVs. We emphasize that the shape of the DPPC liposomes changes from that of MLVs to LUVs by the mixing of a small amount of SA into the DPPC liposomes.

KW - freeze-fracture electron microscopy

KW - large unilamellar vesicle

KW - mixed liposome

KW - stearylamine

KW - trapping efficiency

UR - http://www.scopus.com/inward/record.url?scp=85015684874&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015684874&partnerID=8YFLogxK

U2 - 10.5650/jos.54.251

DO - 10.5650/jos.54.251

M3 - Article

SN - 1345-8957

VL - 54

SP - 251

EP - 254

JO - Journal of Oleo Science

JF - Journal of Oleo Science

IS - 4

ER -

Stearylamine Changes the Liposomal Shape from MLVs to LUVs

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this