Stearylamine Changes the Liposomal Shape from MLVs to LUVs

Shoko Yokoyama, Akiko Inagaki, Koji Tsuchiya, Hideki Sakai, Tomohiro Imura, Takahiro Ohkubo, Nobuyuki Tsubaki, Masahiko Abe

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The shape of mixed dipalmitoylphosphatidylcholine (DPPC)/stearylamine (SA) liposomes prepared by the Bangham method was examined using freeze-fracture electron microscopy. The shape of the mixed DPPC/SA liposomes (XSA = 0.05) was not that of multilamellar vesicles (MLVs) but large unilamellar vesicles (LUVs) with a diameter of approximately 1.0 μm. These facts overthrow the preconceived idea that the shape of the DPPC/SA liposomes prepared by the Bangham method is that of MLVs. The trapping efficiency of the mixed DPPC/SA liposomes (XSA = 0.05) was ten times greater than that of the DPPC liposomes, since the DPPC liposomes were MLVs. The data of the DSC peak for the DPPC/SA liposomes also indicated that the shape of the DPPC/SA liposomes is that of LUVs. We emphasize that the shape of the DPPC liposomes changes from that of MLVs to LUVs by the mixing of a small amount of SA into the DPPC liposomes.

Original languageEnglish
Pages (from-to)251-254
Number of pages4
JournalJournal of Oleo Science
Volume54
Issue number4
DOIs
Publication statusPublished - 2005
Externally publishedYes

Keywords

  • freeze-fracture electron microscopy
  • large unilamellar vesicle
  • mixed liposome
  • stearylamine
  • trapping efficiency

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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