Abstract
Corpus luteum (CL) regression is required during the estrous cycle. During CL regression, luteal cells stop producing progesterone and are degraded by apoptosis. However, the detailed mechanism of CL regression in cattle has not been fully elucidated. The aim of this study was to evaluate autophagy, lysosome activity, and apoptosis during CL regression in cattle. The expression of autophagy-related genes (LC3α, LC3β, Atg3, and Atg7) and the protein LC3-II was significantly higher in the late CL than in the mid CL. In addition, autophagy activity was significantly increased in the late CL. Moreover, gene expression of the autophagy inhibitor mammalian target of rapamycin (mTOR) was significantly lower in the late CL than in the mid CL. Lysosome activation and expression of cathepsin-related genes (CTSB, CTSD, and CTSZ) showed significant increases in the late CL and were associated with an increase in cathepsin B protein. In addition, mRNA expression and activity of caspase 3 (CASP3), an apoptotic enzyme, were significantly higher in the late CL than in the mid CL. These results suggest simultaneous upregulation of autophagy-related factors, lysosomal enzymes and apoptotic mediators, which are involved in regression of the bovine CL.
| Original language | English |
|---|---|
| Pages (from-to) | 229-236 |
| Number of pages | 8 |
| Journal | Journal of Reproduction and Development |
| Volume | 61 |
| Issue number | 3 |
| Publication status | Published - 2015 |
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Keywords
- Apoptosis
- Autophagy
- Cathepsin
- Corpus luteum regression
- LC3
- Lysosome
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle. / Aboelenain, Mansour; Kawahara, Manabu; Balboula, Ahmed Zaky; Montasser, Abd El Monem; Zaabel, Samy Mowaed; Okuda, Kiyoshi; Takahashi, Masashi.
In: Journal of Reproduction and Development, Vol. 61, No. 3, 2015, p. 229-236.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle
AU - Aboelenain, Mansour
AU - Kawahara, Manabu
AU - Balboula, Ahmed Zaky
AU - Montasser, Abd El Monem
AU - Zaabel, Samy Mowaed
AU - Okuda, Kiyoshi
AU - Takahashi, Masashi
PY - 2015
Y1 - 2015
N2 - Corpus luteum (CL) regression is required during the estrous cycle. During CL regression, luteal cells stop producing progesterone and are degraded by apoptosis. However, the detailed mechanism of CL regression in cattle has not been fully elucidated. The aim of this study was to evaluate autophagy, lysosome activity, and apoptosis during CL regression in cattle. The expression of autophagy-related genes (LC3α, LC3β, Atg3, and Atg7) and the protein LC3-II was significantly higher in the late CL than in the mid CL. In addition, autophagy activity was significantly increased in the late CL. Moreover, gene expression of the autophagy inhibitor mammalian target of rapamycin (mTOR) was significantly lower in the late CL than in the mid CL. Lysosome activation and expression of cathepsin-related genes (CTSB, CTSD, and CTSZ) showed significant increases in the late CL and were associated with an increase in cathepsin B protein. In addition, mRNA expression and activity of caspase 3 (CASP3), an apoptotic enzyme, were significantly higher in the late CL than in the mid CL. These results suggest simultaneous upregulation of autophagy-related factors, lysosomal enzymes and apoptotic mediators, which are involved in regression of the bovine CL.
AB - Corpus luteum (CL) regression is required during the estrous cycle. During CL regression, luteal cells stop producing progesterone and are degraded by apoptosis. However, the detailed mechanism of CL regression in cattle has not been fully elucidated. The aim of this study was to evaluate autophagy, lysosome activity, and apoptosis during CL regression in cattle. The expression of autophagy-related genes (LC3α, LC3β, Atg3, and Atg7) and the protein LC3-II was significantly higher in the late CL than in the mid CL. In addition, autophagy activity was significantly increased in the late CL. Moreover, gene expression of the autophagy inhibitor mammalian target of rapamycin (mTOR) was significantly lower in the late CL than in the mid CL. Lysosome activation and expression of cathepsin-related genes (CTSB, CTSD, and CTSZ) showed significant increases in the late CL and were associated with an increase in cathepsin B protein. In addition, mRNA expression and activity of caspase 3 (CASP3), an apoptotic enzyme, were significantly higher in the late CL than in the mid CL. These results suggest simultaneous upregulation of autophagy-related factors, lysosomal enzymes and apoptotic mediators, which are involved in regression of the bovine CL.
KW - Apoptosis
KW - Autophagy
KW - Cathepsin
KW - Corpus luteum regression
KW - LC3
KW - Lysosome
UR - http://www.scopus.com/inward/record.url?scp=84932092315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84932092315&partnerID=8YFLogxK
M3 - Article
VL - 61
SP - 229
EP - 236
JO - Journal of Reproduction and Development
JF - Journal of Reproduction and Development
SN - 0916-8818
IS - 3
ER -