State transition mechanism in arabidopsis thaliana: Biophysical and proteomic studies

Sreedhar Nellaepalli, Ottó Zsiros, László Kovács, Yadavalli Venkateswarlu, Mekala Nageswara Rao, Prasanna Mohanty, Rajagopal Subramanyam

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The redox state of plastoquinone (PQ) pool is the incipient signal in the signal transduction pathway of state transition mechanism, shifting from state I to state II and vice versa. The redox state of the QA, the primary acceptor of photosystem II (PSII) and the PQ pool are easily monitored by the OJIP fast fluorescence transients. The OJIP fast Chl afluorescence transient studies revealed that in state II, there was reduction in maximal fluorescence which could be due to decreased antennae size of PSII. The same changes were not observed in Stn7 mutant lacking thylakoid kinase which phosphorylates light harvesting complex (LHC) II. The phosphorylated LHCII is associated with PSI under state II condition. The redox state of PQ pool is signal for the kinase to phosphorylate/dephosphorylate major LHCII of PSII. The 2-D electrophoresis results showed that LHCII is resolved into 3 spots in state I. However, in state II this has been resolved into 4 spots. However, Stn7 mutant there was no change of 2D spots in state II. The additional spot is yet to be investigated.

Original languageEnglish
Title of host publicationAdvanced Topics in Science and Technology in China
PublisherSpringer
Pages398-401
Number of pages4
DOIs
Publication statusPublished - Jan 1 2013
Externally publishedYes

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

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Keywords

  • 2D electrophoresis
  • Arabidopsis thaliana
  • Chl a fluorescence
  • Photosystems
  • State transition

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Engineering(all)
  • General

Cite this

Nellaepalli, S., Zsiros, O., Kovács, L., Venkateswarlu, Y., Rao, M. N., Mohanty, P., & Subramanyam, R. (2013). State transition mechanism in arabidopsis thaliana: Biophysical and proteomic studies. In Advanced Topics in Science and Technology in China (pp. 398-401). (Advanced Topics in Science and Technology in China). Springer. https://doi.org/10.1007/978-3-642-32034-7_83