State transition mechanism in arabidopsis thaliana: Biophysical and proteomic studies

Sreedhar Nellaepalli, Ottó Zsiros, László Kovács, Yadavalli Venkateswarlu, Mekala Nageswara Rao, Prasanna Mohanty, Rajagopal Subramanyam

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The redox state of plastoquinone (PQ) pool is the incipient signal in the signal transduction pathway of state transition mechanism, shifting from state I to state II and vice versa. The redox state of the QA, the primary acceptor of photosystem II (PSII) and the PQ pool are easily monitored by the OJIP fast fluorescence transients. The OJIP fast Chl afluorescence transient studies revealed that in state II, there was reduction in maximal fluorescence which could be due to decreased antennae size of PSII. The same changes were not observed in Stn7 mutant lacking thylakoid kinase which phosphorylates light harvesting complex (LHC) II. The phosphorylated LHCII is associated with PSI under state II condition. The redox state of PQ pool is signal for the kinase to phosphorylate/dephosphorylate major LHCII of PSII. The 2-D electrophoresis results showed that LHCII is resolved into 3 spots in state I. However, in state II this has been resolved into 4 spots. However, Stn7 mutant there was no change of 2D spots in state II. The additional spot is yet to be investigated.

Original languageEnglish
Title of host publicationAdvanced Topics in Science and Technology in China
PublisherSpringer
Pages398-401
Number of pages4
DOIs
Publication statusPublished - Jan 1 2013
Externally publishedYes

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

Fingerprint

Plastoquinone
Photosystem II Protein Complex
Phosphotransferases
Fluorescence
Signal transduction
Electrophoresis
Antennas
Oxidation-Reduction
Proteomics

Keywords

  • 2D electrophoresis
  • Arabidopsis thaliana
  • Chl a fluorescence
  • Photosystems
  • State transition

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Engineering(all)
  • General

Cite this

Nellaepalli, S., Zsiros, O., Kovács, L., Venkateswarlu, Y., Rao, M. N., Mohanty, P., & Subramanyam, R. (2013). State transition mechanism in arabidopsis thaliana: Biophysical and proteomic studies. In Advanced Topics in Science and Technology in China (pp. 398-401). (Advanced Topics in Science and Technology in China). Springer. https://doi.org/10.1007/978-3-642-32034-7_83

State transition mechanism in arabidopsis thaliana : Biophysical and proteomic studies. / Nellaepalli, Sreedhar; Zsiros, Ottó; Kovács, László; Venkateswarlu, Yadavalli; Rao, Mekala Nageswara; Mohanty, Prasanna; Subramanyam, Rajagopal.

Advanced Topics in Science and Technology in China. Springer, 2013. p. 398-401 (Advanced Topics in Science and Technology in China).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nellaepalli, S, Zsiros, O, Kovács, L, Venkateswarlu, Y, Rao, MN, Mohanty, P & Subramanyam, R 2013, State transition mechanism in arabidopsis thaliana: Biophysical and proteomic studies. in Advanced Topics in Science and Technology in China. Advanced Topics in Science and Technology in China, Springer, pp. 398-401. https://doi.org/10.1007/978-3-642-32034-7_83
Nellaepalli S, Zsiros O, Kovács L, Venkateswarlu Y, Rao MN, Mohanty P et al. State transition mechanism in arabidopsis thaliana: Biophysical and proteomic studies. In Advanced Topics in Science and Technology in China. Springer. 2013. p. 398-401. (Advanced Topics in Science and Technology in China). https://doi.org/10.1007/978-3-642-32034-7_83
Nellaepalli, Sreedhar ; Zsiros, Ottó ; Kovács, László ; Venkateswarlu, Yadavalli ; Rao, Mekala Nageswara ; Mohanty, Prasanna ; Subramanyam, Rajagopal. / State transition mechanism in arabidopsis thaliana : Biophysical and proteomic studies. Advanced Topics in Science and Technology in China. Springer, 2013. pp. 398-401 (Advanced Topics in Science and Technology in China).
@inbook{3bdab92e6ec14ac1935a2ead7588dc8f,
title = "State transition mechanism in arabidopsis thaliana: Biophysical and proteomic studies",
abstract = "The redox state of plastoquinone (PQ) pool is the incipient signal in the signal transduction pathway of state transition mechanism, shifting from state I to state II and vice versa. The redox state of the QA, the primary acceptor of photosystem II (PSII) and the PQ pool are easily monitored by the OJIP fast fluorescence transients. The OJIP fast Chl afluorescence transient studies revealed that in state II, there was reduction in maximal fluorescence which could be due to decreased antennae size of PSII. The same changes were not observed in Stn7 mutant lacking thylakoid kinase which phosphorylates light harvesting complex (LHC) II. The phosphorylated LHCII is associated with PSI under state II condition. The redox state of PQ pool is signal for the kinase to phosphorylate/dephosphorylate major LHCII of PSII. The 2-D electrophoresis results showed that LHCII is resolved into 3 spots in state I. However, in state II this has been resolved into 4 spots. However, Stn7 mutant there was no change of 2D spots in state II. The additional spot is yet to be investigated.",
keywords = "2D electrophoresis, Arabidopsis thaliana, Chl a fluorescence, Photosystems, State transition",
author = "Sreedhar Nellaepalli and Ott{\'o} Zsiros and L{\'a}szl{\'o} Kov{\'a}cs and Yadavalli Venkateswarlu and Rao, {Mekala Nageswara} and Prasanna Mohanty and Rajagopal Subramanyam",
year = "2013",
month = "1",
day = "1",
doi = "10.1007/978-3-642-32034-7_83",
language = "English",
series = "Advanced Topics in Science and Technology in China",
publisher = "Springer",
pages = "398--401",
booktitle = "Advanced Topics in Science and Technology in China",

}

TY - CHAP

T1 - State transition mechanism in arabidopsis thaliana

T2 - Biophysical and proteomic studies

AU - Nellaepalli, Sreedhar

AU - Zsiros, Ottó

AU - Kovács, László

AU - Venkateswarlu, Yadavalli

AU - Rao, Mekala Nageswara

AU - Mohanty, Prasanna

AU - Subramanyam, Rajagopal

PY - 2013/1/1

Y1 - 2013/1/1

N2 - The redox state of plastoquinone (PQ) pool is the incipient signal in the signal transduction pathway of state transition mechanism, shifting from state I to state II and vice versa. The redox state of the QA, the primary acceptor of photosystem II (PSII) and the PQ pool are easily monitored by the OJIP fast fluorescence transients. The OJIP fast Chl afluorescence transient studies revealed that in state II, there was reduction in maximal fluorescence which could be due to decreased antennae size of PSII. The same changes were not observed in Stn7 mutant lacking thylakoid kinase which phosphorylates light harvesting complex (LHC) II. The phosphorylated LHCII is associated with PSI under state II condition. The redox state of PQ pool is signal for the kinase to phosphorylate/dephosphorylate major LHCII of PSII. The 2-D electrophoresis results showed that LHCII is resolved into 3 spots in state I. However, in state II this has been resolved into 4 spots. However, Stn7 mutant there was no change of 2D spots in state II. The additional spot is yet to be investigated.

AB - The redox state of plastoquinone (PQ) pool is the incipient signal in the signal transduction pathway of state transition mechanism, shifting from state I to state II and vice versa. The redox state of the QA, the primary acceptor of photosystem II (PSII) and the PQ pool are easily monitored by the OJIP fast fluorescence transients. The OJIP fast Chl afluorescence transient studies revealed that in state II, there was reduction in maximal fluorescence which could be due to decreased antennae size of PSII. The same changes were not observed in Stn7 mutant lacking thylakoid kinase which phosphorylates light harvesting complex (LHC) II. The phosphorylated LHCII is associated with PSI under state II condition. The redox state of PQ pool is signal for the kinase to phosphorylate/dephosphorylate major LHCII of PSII. The 2-D electrophoresis results showed that LHCII is resolved into 3 spots in state I. However, in state II this has been resolved into 4 spots. However, Stn7 mutant there was no change of 2D spots in state II. The additional spot is yet to be investigated.

KW - 2D electrophoresis

KW - Arabidopsis thaliana

KW - Chl a fluorescence

KW - Photosystems

KW - State transition

UR - http://www.scopus.com/inward/record.url?scp=85060718500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060718500&partnerID=8YFLogxK

U2 - 10.1007/978-3-642-32034-7_83

DO - 10.1007/978-3-642-32034-7_83

M3 - Chapter

AN - SCOPUS:85060718500

T3 - Advanced Topics in Science and Technology in China

SP - 398

EP - 401

BT - Advanced Topics in Science and Technology in China

PB - Springer

ER -