Staining air dried protoplasts for study of plant chromosomes

M. Murata

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The air drying technique used in mammalian cytology was applied to isolated plant protoplasts for study of chromosomes. For cultured celery cells, this technique resulted in good spreads of metaphase chromosomes with high resolution. Mitotic chromosomes of Brassica species are relatively small, poorly stained by common stains, and difficult to spread by the squash technique. In this study, however, the chromosomes of B. carinata in callus culture were spread well and stained clearly with Giemsa staining solution. The chromosome preparations by the present techniques should also be amenable to chromosome banding studies in plants.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalBiotechnic and Histochemistry
Volume58
Issue number2
DOIs
Publication statusPublished - Jan 1 1983

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

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