Staining air dried protoplasts for study of plant chromosomes

M. Murata

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The air drying technique used in mammalian cytology was applied to isolated plant protoplasts for study of chromosomes. For cultured celery cells, this technique resulted in good spreads of metaphase chromosomes with high resolution. Mitotic chromosomes of Brassica species are relatively small, poorly stained by common stains, and difficult to spread by the squash technique. In this study, however, the chromosomes of B. carinata in callus culture were spread well and stained clearly with Giemsa staining solution. The chromosome preparations by the present techniques should also be amenable to chromosome banding studies in plants.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalStain Technology
Issue number2
Publication statusPublished - 1983
Externally publishedYes


ASJC Scopus subject areas

  • Anatomy

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