Staining air dried protoplasts for study of plant chromosomes

M. Murata

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The air drying technique used in mammalian cytology was applied to isolated plant protoplasts for study of chromosomes. For cultured celery cells, this technique resulted in good spreads of metaphase chromosomes with high resolution. Mitotic chromosomes of Brassica species are relatively small, poorly stained by common stains, and difficult to spread by the squash technique. In this study, however, the chromosomes of B. carinata in callus culture were spread well and stained clearly with Giemsa staining solution. The chromosome preparations by the present techniques should also be amenable to chromosome banding studies in plants.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalStain Technology
Volume58
Issue number2
DOIs
Publication statusPublished - 1983
Externally publishedYes

Fingerprint

Plant Chromosomes
Protoplasts
Chromosomes
Air
Staining and Labeling
Apium graveolens
Chromosomes, Human, 4-5
Chromosome Banding
Cucurbita
Brassica
Bony Callus
Metaphase
Cell Biology
Cultured Cells
Coloring Agents

ASJC Scopus subject areas

  • Anatomy

Cite this

Staining air dried protoplasts for study of plant chromosomes. / Murata, M.

In: Stain Technology, Vol. 58, No. 2, 1983, p. 101-106.

Research output: Contribution to journalArticle

Murata, M. / Staining air dried protoplasts for study of plant chromosomes. In: Stain Technology. 1983 ; Vol. 58, No. 2. pp. 101-106.
@article{bec8d84ca9b342e0880dc5ce572f0871,
title = "Staining air dried protoplasts for study of plant chromosomes",
abstract = "The air drying technique used in mammalian cytology was applied to isolated plant protoplasts for study of chromosomes. For cultured celery cells, this technique resulted in good spreads of metaphase chromosomes with high resolution. Mitotic chromosomes of Brassica species are relatively small, poorly stained by common stains, and difficult to spread by the squash technique. In this study, however, the chromosomes of B. carinata in callus culture were spread well and stained clearly with Giemsa staining solution. The chromosome preparations by the present techniques should also be amenable to chromosome banding studies in plants.",
author = "M. Murata",
year = "1983",
doi = "10.3109/10520298309066762",
language = "English",
volume = "58",
pages = "101--106",
journal = "Biotechnic and Histochemistry",
issn = "1052-0295",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Staining air dried protoplasts for study of plant chromosomes

AU - Murata, M.

PY - 1983

Y1 - 1983

N2 - The air drying technique used in mammalian cytology was applied to isolated plant protoplasts for study of chromosomes. For cultured celery cells, this technique resulted in good spreads of metaphase chromosomes with high resolution. Mitotic chromosomes of Brassica species are relatively small, poorly stained by common stains, and difficult to spread by the squash technique. In this study, however, the chromosomes of B. carinata in callus culture were spread well and stained clearly with Giemsa staining solution. The chromosome preparations by the present techniques should also be amenable to chromosome banding studies in plants.

AB - The air drying technique used in mammalian cytology was applied to isolated plant protoplasts for study of chromosomes. For cultured celery cells, this technique resulted in good spreads of metaphase chromosomes with high resolution. Mitotic chromosomes of Brassica species are relatively small, poorly stained by common stains, and difficult to spread by the squash technique. In this study, however, the chromosomes of B. carinata in callus culture were spread well and stained clearly with Giemsa staining solution. The chromosome preparations by the present techniques should also be amenable to chromosome banding studies in plants.

UR - http://www.scopus.com/inward/record.url?scp=0021089502&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021089502&partnerID=8YFLogxK

U2 - 10.3109/10520298309066762

DO - 10.3109/10520298309066762

M3 - Article

VL - 58

SP - 101

EP - 106

JO - Biotechnic and Histochemistry

JF - Biotechnic and Histochemistry

SN - 1052-0295

IS - 2

ER -