Stable isotope labeling of Arabidopsis thaliana for an NMR-based metabolomics approach

Jun Kikuchi, Kazuo Shinozaki, Takashi Hirayama

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Nuclear magnetic resonance (NMR) will become a key technology in plant metabolomics with the use of stable isotope labeling and advanced hetero-nuclear NMR methodologies. To demonstrate the power of this approach, we performed multi-dimensional hetero-nuclear NMR analysis of metabolic movement of carbon and nitrogen nuclei in Arabidopsis thaliana. First, distinct ethanol-stress response was investigated using 13C-labeled wild type and an ethanol-hypersensitive mutant plants. Furthermore, we followed nitrogen fluxes in 15N-labeled seeds during the initiation of germination in vivo. The future role of stable isotope-labeling combined with advanced hetero-nuclear NMR in plant metabolomics is discussed.

Original languageEnglish
Pages (from-to)1099-1104
Number of pages6
JournalPlant and Cell Physiology
Volume45
Issue number8
DOIs
Publication statusPublished - Aug 2004
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • Nuclear magnetic resonance
  • Plant metabolomics
  • Stable isotope labeling

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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