Stable expression of human β1,4-galactosyltransferase in plant cells modifies N-linked glycosylation patterns

Nirianne Q. Palacpac, Shohei Yoshida, Hiromi Sakai, Yoshinobu Kimura, Kazuhito Fujiyama, Toshiomi Yoshida, Tatsuji Seki

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

β1,4-Galactosyltransferase (UDP galactose: β-N-acetylglucosaminide: β1,4-galactosyltransferase; EC 2.4.1.22) catalyzes the transfer of galactose from UDP-Gal to N-acetylglucosamine in the penultimate stages of the terminal glycosylation of N-linked complex oligosaccharides in mammalian cells. Tobacco BY2 cells lack this Golgi enzyme. To determine to what extent the production of a mammalian glycosyltransferase can alter the glycosylation pathway of plant cells, tobacco BY2 suspension-cultured cells were stably transformed with the full-length human galactosyltransferase gene placed under the control of the cauliflower mosaic virus 35S promoter. The expression was confirmed by assaying enzymatic activity as well as by Southern and Western blotting. The transformant with the highest level of enzymatic activity has glycans with galactose residues at the terminal nonreducing ends, indicating the successful modification of the plant cell N- glycosylation pathway. Analysis of the oligosaccharide structures shows that the galactosylated N-glycans account for 47.3% of the total sugar chains. In addition, the absence of the dominant xylosidated- and fucosylated-type sugar chains confirms that the transformed cells can be used to produce glycoproteins without the highly immunogenic glycans typically found in plants. These results demonstrate the synthesis in plants of N-linked glycans with modified and defined sugar chain structures similar to mammalian glycoproteins.

Original languageEnglish
Pages (from-to)4692-4697
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number8
DOIs
Publication statusPublished - Apr 13 1999

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Galactosyltransferases
Plant Cells
Glycosylation
Polysaccharides
Uridine Diphosphate Galactose
Oligosaccharides
Tobacco
Lactose Synthase
Glycoproteins
Caulimovirus
Glycosyltransferases
Acetylglucosamine
Southern Blotting
Galactose
Cultured Cells
Suspensions
Western Blotting
Enzymes
Genes

ASJC Scopus subject areas

  • Genetics
  • General

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Stable expression of human β1,4-galactosyltransferase in plant cells modifies N-linked glycosylation patterns. / Palacpac, Nirianne Q.; Yoshida, Shohei; Sakai, Hiromi; Kimura, Yoshinobu; Fujiyama, Kazuhito; Yoshida, Toshiomi; Seki, Tatsuji.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 8, 13.04.1999, p. 4692-4697.

Research output: Contribution to journalArticle

Palacpac, Nirianne Q. ; Yoshida, Shohei ; Sakai, Hiromi ; Kimura, Yoshinobu ; Fujiyama, Kazuhito ; Yoshida, Toshiomi ; Seki, Tatsuji. / Stable expression of human β1,4-galactosyltransferase in plant cells modifies N-linked glycosylation patterns. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 8. pp. 4692-4697.
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