Stabilization of the oxygen-evolving complex of photosystem II by bicarbonate and glycinebetaine in thylakoid and subthylakoid preparations

Vyacheslav V. Klimov, Suleyman Allakhverdiev, Yoshitaka Nishiyama, Andrei A. Khorobrykh, Norio Murata

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The protective effect of 1 M glycinebetaine on thermal inactivation of photosynthetic oxygen evolution in isolated photosystem II membrane fragments from spinach is observed in CO2-free medium in both the presence and absence of added 2 mM bicarbonate. Conversely, the protective effect of 2 mM bicarbonate against thermoinactivation is seen in the absence as well as in the presence of 1 M glycinebetaine. The stabilizing effect of bicarbonate is also observed in thylakoid membranes from Synechococcus sp. PCC 7002 treated with 0.1% Triton X-100, and in unbroken spinach thylakoids. It is shown for the first time that bicarbonate protects the water-oxidizing complex against inactivation induced by pre-incubation of photosystem II membrane fragments (25°C) and thylakoids (40°C) at low pH (5.0-5.5) in non-bicarbonate-depleted medium. We conclude that the protective effects of glycinebetaine and bicarbonate are of a different nature; glycinebetaine acts as a non-specific, compatible, zwitterionic osmolyte while bicarbonate is considered an essential constituent of the water-oxidizing complex of photosystem II, important for its functioning and stabilization.

Original languageEnglish
Pages (from-to)797-803
Number of pages7
JournalFunctional Plant Biology
Volume30
Issue number7
DOIs
Publication statusPublished - Sep 11 2003
Externally publishedYes

Fingerprint

oxygen evolving complex
betaine
bicarbonates
thylakoids
photosystem II
protective effect
spinach
Synechococcus sp. PCC 7002
heat inactivation
inactivation
water
carbon dioxide

Keywords

  • Bicarbonate
  • Glycinebetaine
  • Photosystem II
  • Thermostability
  • Water-oxidizing complex

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Stabilization of the oxygen-evolving complex of photosystem II by bicarbonate and glycinebetaine in thylakoid and subthylakoid preparations. / Klimov, Vyacheslav V.; Allakhverdiev, Suleyman; Nishiyama, Yoshitaka; Khorobrykh, Andrei A.; Murata, Norio.

In: Functional Plant Biology, Vol. 30, No. 7, 11.09.2003, p. 797-803.

Research output: Contribution to journalArticle

Klimov, Vyacheslav V. ; Allakhverdiev, Suleyman ; Nishiyama, Yoshitaka ; Khorobrykh, Andrei A. ; Murata, Norio. / Stabilization of the oxygen-evolving complex of photosystem II by bicarbonate and glycinebetaine in thylakoid and subthylakoid preparations. In: Functional Plant Biology. 2003 ; Vol. 30, No. 7. pp. 797-803.
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