Abstract
The protective effect of 1 M glycinebetaine on thermal inactivation of photosynthetic oxygen evolution in isolated photosystem II membrane fragments from spinach is observed in CO2-free medium in both the presence and absence of added 2 mM bicarbonate. Conversely, the protective effect of 2 mM bicarbonate against thermoinactivation is seen in the absence as well as in the presence of 1 M glycinebetaine. The stabilizing effect of bicarbonate is also observed in thylakoid membranes from Synechococcus sp. PCC 7002 treated with 0.1% Triton X-100, and in unbroken spinach thylakoids. It is shown for the first time that bicarbonate protects the water-oxidizing complex against inactivation induced by pre-incubation of photosystem II membrane fragments (25°C) and thylakoids (40°C) at low pH (5.0-5.5) in non-bicarbonate-depleted medium. We conclude that the protective effects of glycinebetaine and bicarbonate are of a different nature; glycinebetaine acts as a non-specific, compatible, zwitterionic osmolyte while bicarbonate is considered an essential constituent of the water-oxidizing complex of photosystem II, important for its functioning and stabilization.
| Original language | English |
|---|---|
| Pages (from-to) | 797-803 |
| Number of pages | 7 |
| Journal | Functional Plant Biology |
| Volume | 30 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Sep 11 2003 |
| Externally published | Yes |
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Keywords
- Bicarbonate
- Glycinebetaine
- Photosystem II
- Thermostability
- Water-oxidizing complex
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science
Cite this
Stabilization of the oxygen-evolving complex of photosystem II by bicarbonate and glycinebetaine in thylakoid and subthylakoid preparations. / Klimov, Vyacheslav V.; Allakhverdiev, Suleyman; Nishiyama, Yoshitaka; Khorobrykh, Andrei A.; Murata, Norio.
In: Functional Plant Biology, Vol. 30, No. 7, 11.09.2003, p. 797-803.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stabilization of the oxygen-evolving complex of photosystem II by bicarbonate and glycinebetaine in thylakoid and subthylakoid preparations
AU - Klimov, Vyacheslav V.
AU - Allakhverdiev, Suleyman
AU - Nishiyama, Yoshitaka
AU - Khorobrykh, Andrei A.
AU - Murata, Norio
PY - 2003/9/11
Y1 - 2003/9/11
N2 - The protective effect of 1 M glycinebetaine on thermal inactivation of photosynthetic oxygen evolution in isolated photosystem II membrane fragments from spinach is observed in CO2-free medium in both the presence and absence of added 2 mM bicarbonate. Conversely, the protective effect of 2 mM bicarbonate against thermoinactivation is seen in the absence as well as in the presence of 1 M glycinebetaine. The stabilizing effect of bicarbonate is also observed in thylakoid membranes from Synechococcus sp. PCC 7002 treated with 0.1% Triton X-100, and in unbroken spinach thylakoids. It is shown for the first time that bicarbonate protects the water-oxidizing complex against inactivation induced by pre-incubation of photosystem II membrane fragments (25°C) and thylakoids (40°C) at low pH (5.0-5.5) in non-bicarbonate-depleted medium. We conclude that the protective effects of glycinebetaine and bicarbonate are of a different nature; glycinebetaine acts as a non-specific, compatible, zwitterionic osmolyte while bicarbonate is considered an essential constituent of the water-oxidizing complex of photosystem II, important for its functioning and stabilization.
AB - The protective effect of 1 M glycinebetaine on thermal inactivation of photosynthetic oxygen evolution in isolated photosystem II membrane fragments from spinach is observed in CO2-free medium in both the presence and absence of added 2 mM bicarbonate. Conversely, the protective effect of 2 mM bicarbonate against thermoinactivation is seen in the absence as well as in the presence of 1 M glycinebetaine. The stabilizing effect of bicarbonate is also observed in thylakoid membranes from Synechococcus sp. PCC 7002 treated with 0.1% Triton X-100, and in unbroken spinach thylakoids. It is shown for the first time that bicarbonate protects the water-oxidizing complex against inactivation induced by pre-incubation of photosystem II membrane fragments (25°C) and thylakoids (40°C) at low pH (5.0-5.5) in non-bicarbonate-depleted medium. We conclude that the protective effects of glycinebetaine and bicarbonate are of a different nature; glycinebetaine acts as a non-specific, compatible, zwitterionic osmolyte while bicarbonate is considered an essential constituent of the water-oxidizing complex of photosystem II, important for its functioning and stabilization.
KW - Bicarbonate
KW - Glycinebetaine
KW - Photosystem II
KW - Thermostability
KW - Water-oxidizing complex
UR - http://www.scopus.com/inward/record.url?scp=0041350429&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041350429&partnerID=8YFLogxK
U2 - 10.1071/FP03068
DO - 10.1071/FP03068
M3 - Article
VL - 30
SP - 797
EP - 803
JO - Functional Plant Biology
JF - Functional Plant Biology
SN - 1445-4408
IS - 7
ER -