SsrA-mediated tagging and proteolysis of Lacl and its role in the regulation of lac operon

Abo Tatsuhiko, Toshifumi Inada, Kazuko Ogawa, Hiroji Aiba

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

SsrA RNA of Escherichia coli, also known as 10Sa RNA or tmRNA, acts both as tRNA and mRNA when ribosomes are paused at the 3' end of an mRNA lacking a stop codon. This process, referred to as trans-translation, leads to the addition of a short peptide tag to the C-terminus of the incomplete nascent polypeptide. The tagged polypeptide is then degraded by C-terminal-specific proteases. Here, we focused on endogenous targets for the SsrA system and on a potential regulatory role of SsrA RNA. First, we show that trans-translation events occur frequently in normally growing E. coli cells. More specifically, we report that the lacI mRNA encoding Lac repressor (LacI) is a specific natural target for trans-translation. The binding of LacI to the lac operators results in truncated lacI mRNAs that are, in turn, recognized by the SsrA system. The SsrA-mediated tagging and proteolysis of LacI appears to play a role in cellular adaptation to lactose availability by supporting a rapid induction of lac operon expression.

Original languageEnglish
Pages (from-to)3762-3769
Number of pages8
JournalEMBO Journal
Volume19
Issue number14
DOIs
Publication statusPublished - Jul 17 2000
Externally publishedYes

Keywords

  • Lac repressor
  • Transcriptional roadblock
  • lac operon
  • tmRNA
  • trans-translation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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