SsrA-mediated tagging and proteolysis of Lacl and its role in the regulation of lac operon

Tatsuhiko Abo, Toshifumi Inada, Kazuko Ogawa, Hiroji Aiba

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

SsrA RNA of Escherichia coli, also known as 10Sa RNA or tmRNA, acts both as tRNA and mRNA when ribosomes are paused at the 3' end of an mRNA lacking a stop codon. This process, referred to as trans-translation, leads to the addition of a short peptide tag to the C-terminus of the incomplete nascent polypeptide. The tagged polypeptide is then degraded by C-terminal-specific proteases. Here, we focused on endogenous targets for the SsrA system and on a potential regulatory role of SsrA RNA. First, we show that trans-translation events occur frequently in normally growing E. coli cells. More specifically, we report that the lacI mRNA encoding Lac repressor (LacI) is a specific natural target for trans-translation. The binding of LacI to the lac operators results in truncated lacI mRNAs that are, in turn, recognized by the SsrA system. The SsrA-mediated tagging and proteolysis of LacI appears to play a role in cellular adaptation to lactose availability by supporting a rapid induction of lac operon expression.

Original languageEnglish
Pages (from-to)3762-3769
Number of pages8
JournalEMBO Journal
Volume19
Issue number14
Publication statusPublished - Jul 17 2000
Externally publishedYes

Fingerprint

Proteolysis
Lac Operon
Messenger RNA
Escherichia coli
Peptides
Lac Repressors
RNA
Terminator Codon
Lactose
Transfer RNA
Ribosomes
Peptide Hydrolases
Availability
tmRNA

Keywords

  • lac operon
  • Lac repressor
  • tmRNA
  • trans-translation
  • Transcriptional roadblock

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

SsrA-mediated tagging and proteolysis of Lacl and its role in the regulation of lac operon. / Abo, Tatsuhiko; Inada, Toshifumi; Ogawa, Kazuko; Aiba, Hiroji.

In: EMBO Journal, Vol. 19, No. 14, 17.07.2000, p. 3762-3769.

Research output: Contribution to journalArticle

Abo, T, Inada, T, Ogawa, K & Aiba, H 2000, 'SsrA-mediated tagging and proteolysis of Lacl and its role in the regulation of lac operon', EMBO Journal, vol. 19, no. 14, pp. 3762-3769.
Abo, Tatsuhiko ; Inada, Toshifumi ; Ogawa, Kazuko ; Aiba, Hiroji. / SsrA-mediated tagging and proteolysis of Lacl and its role in the regulation of lac operon. In: EMBO Journal. 2000 ; Vol. 19, No. 14. pp. 3762-3769.
@article{7259bcaf92d14e6383425939465e3cca,
title = "SsrA-mediated tagging and proteolysis of Lacl and its role in the regulation of lac operon",
abstract = "SsrA RNA of Escherichia coli, also known as 10Sa RNA or tmRNA, acts both as tRNA and mRNA when ribosomes are paused at the 3' end of an mRNA lacking a stop codon. This process, referred to as trans-translation, leads to the addition of a short peptide tag to the C-terminus of the incomplete nascent polypeptide. The tagged polypeptide is then degraded by C-terminal-specific proteases. Here, we focused on endogenous targets for the SsrA system and on a potential regulatory role of SsrA RNA. First, we show that trans-translation events occur frequently in normally growing E. coli cells. More specifically, we report that the lacI mRNA encoding Lac repressor (LacI) is a specific natural target for trans-translation. The binding of LacI to the lac operators results in truncated lacI mRNAs that are, in turn, recognized by the SsrA system. The SsrA-mediated tagging and proteolysis of LacI appears to play a role in cellular adaptation to lactose availability by supporting a rapid induction of lac operon expression.",
keywords = "lac operon, Lac repressor, tmRNA, trans-translation, Transcriptional roadblock",
author = "Tatsuhiko Abo and Toshifumi Inada and Kazuko Ogawa and Hiroji Aiba",
year = "2000",
month = "7",
day = "17",
language = "English",
volume = "19",
pages = "3762--3769",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "14",

}

TY - JOUR

T1 - SsrA-mediated tagging and proteolysis of Lacl and its role in the regulation of lac operon

AU - Abo, Tatsuhiko

AU - Inada, Toshifumi

AU - Ogawa, Kazuko

AU - Aiba, Hiroji

PY - 2000/7/17

Y1 - 2000/7/17

N2 - SsrA RNA of Escherichia coli, also known as 10Sa RNA or tmRNA, acts both as tRNA and mRNA when ribosomes are paused at the 3' end of an mRNA lacking a stop codon. This process, referred to as trans-translation, leads to the addition of a short peptide tag to the C-terminus of the incomplete nascent polypeptide. The tagged polypeptide is then degraded by C-terminal-specific proteases. Here, we focused on endogenous targets for the SsrA system and on a potential regulatory role of SsrA RNA. First, we show that trans-translation events occur frequently in normally growing E. coli cells. More specifically, we report that the lacI mRNA encoding Lac repressor (LacI) is a specific natural target for trans-translation. The binding of LacI to the lac operators results in truncated lacI mRNAs that are, in turn, recognized by the SsrA system. The SsrA-mediated tagging and proteolysis of LacI appears to play a role in cellular adaptation to lactose availability by supporting a rapid induction of lac operon expression.

AB - SsrA RNA of Escherichia coli, also known as 10Sa RNA or tmRNA, acts both as tRNA and mRNA when ribosomes are paused at the 3' end of an mRNA lacking a stop codon. This process, referred to as trans-translation, leads to the addition of a short peptide tag to the C-terminus of the incomplete nascent polypeptide. The tagged polypeptide is then degraded by C-terminal-specific proteases. Here, we focused on endogenous targets for the SsrA system and on a potential regulatory role of SsrA RNA. First, we show that trans-translation events occur frequently in normally growing E. coli cells. More specifically, we report that the lacI mRNA encoding Lac repressor (LacI) is a specific natural target for trans-translation. The binding of LacI to the lac operators results in truncated lacI mRNAs that are, in turn, recognized by the SsrA system. The SsrA-mediated tagging and proteolysis of LacI appears to play a role in cellular adaptation to lactose availability by supporting a rapid induction of lac operon expression.

KW - lac operon

KW - Lac repressor

KW - tmRNA

KW - trans-translation

KW - Transcriptional roadblock

UR - http://www.scopus.com/inward/record.url?scp=0343627965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343627965&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 3762

EP - 3769

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 14

ER -