SSEA-4 is a marker of human deciduous periodontal ligament stem cells

H. Fukushima, Noriaki Kawanabe, S. Murata, Yoshihito Ishihara, T. Yanagita, T. A. Balam, T. Yamashiro

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although human deciduous teeth are an ideal source of adult stem cells, no method for identifying deciduous periodontal ligament (D-PDL) stem cells has so far been developed. In the present study, we investigated whether stage-specific embryonic antigen (SSEA)-4 is a marker that could be used to isolate D-PDL stem cells. The isolated D-PDL cells met the minimum criteria for mesenchymal stem cells (MSCs): They showed plastic adherence, specific-surface antigen expression, and multipotent differentiation potential. SSEA-4+ D-PDL cells were detected in vitro and in vivo. A flow cytometric analysis demonstrated that 22.7% of the D-PDL cells were positive for SSEA-4. SSEA-4+ clonal D-PDL cells displayed multilineage differentiation potential: They were able to differentiate into adipocytes, osteoblasts, and chondrocytes in vitro. A clonal assay demonstrated that 61.5% of the SSEA-4+ D-PDL cells had adipogenic, osteogenic, and chondrogenic potential. Our present study demonstrated that SSEA-4+ D-PDL cells are a subset of multipotent stem cells. Hence, SSEA-4 is a specific marker that can be used to identify D-PDL stem cells.

Original languageEnglish
Pages (from-to)955-960
Number of pages6
JournalJournal of Dental Research
Volume91
Issue number10
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Periodontal Ligament
Stem Cells
Multipotent Stem Cells
stage-specific embryonic antigen-4
Adult Stem Cells
Deciduous Tooth
Surface Antigens
Chondrocytes
Osteoblasts
Mesenchymal Stromal Cells
Adipocytes
Plastics

Keywords

  • adult stem cells
  • clonal assays
  • flow cytometry
  • human deciduous periodontal ligament
  • multipotential differentiation
  • stage-specific embryonic antigen-4

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

SSEA-4 is a marker of human deciduous periodontal ligament stem cells. / Fukushima, H.; Kawanabe, Noriaki; Murata, S.; Ishihara, Yoshihito; Yanagita, T.; Balam, T. A.; Yamashiro, T.

In: Journal of Dental Research, Vol. 91, No. 10, 10.2012, p. 955-960.

Research output: Contribution to journalArticle

Fukushima, H. ; Kawanabe, Noriaki ; Murata, S. ; Ishihara, Yoshihito ; Yanagita, T. ; Balam, T. A. ; Yamashiro, T. / SSEA-4 is a marker of human deciduous periodontal ligament stem cells. In: Journal of Dental Research. 2012 ; Vol. 91, No. 10. pp. 955-960.
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