Abstract
Although human deciduous teeth are an ideal source of adult stem cells, no method for identifying deciduous periodontal ligament (D-PDL) stem cells has so far been developed. In the present study, we investigated whether stage-specific embryonic antigen (SSEA)-4 is a marker that could be used to isolate D-PDL stem cells. The isolated D-PDL cells met the minimum criteria for mesenchymal stem cells (MSCs): They showed plastic adherence, specific-surface antigen expression, and multipotent differentiation potential. SSEA-4+ D-PDL cells were detected in vitro and in vivo. A flow cytometric analysis demonstrated that 22.7% of the D-PDL cells were positive for SSEA-4. SSEA-4+ clonal D-PDL cells displayed multilineage differentiation potential: They were able to differentiate into adipocytes, osteoblasts, and chondrocytes in vitro. A clonal assay demonstrated that 61.5% of the SSEA-4+ D-PDL cells had adipogenic, osteogenic, and chondrogenic potential. Our present study demonstrated that SSEA-4+ D-PDL cells are a subset of multipotent stem cells. Hence, SSEA-4 is a specific marker that can be used to identify D-PDL stem cells.
| Original language | English |
|---|---|
| Pages (from-to) | 955-960 |
| Number of pages | 6 |
| Journal | Journal of Dental Research |
| Volume | 91 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2012 |
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Keywords
- adult stem cells
- clonal assays
- flow cytometry
- human deciduous periodontal ligament
- multipotential differentiation
- stage-specific embryonic antigen-4
ASJC Scopus subject areas
- Dentistry(all)
Cite this
SSEA-4 is a marker of human deciduous periodontal ligament stem cells. / Fukushima, H.; Kawanabe, Noriaki; Murata, S.; Ishihara, Yoshihito; Yanagita, T.; Balam, T. A.; Yamashiro, T.
In: Journal of Dental Research, Vol. 91, No. 10, 10.2012, p. 955-960.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - SSEA-4 is a marker of human deciduous periodontal ligament stem cells
AU - Fukushima, H.
AU - Kawanabe, Noriaki
AU - Murata, S.
AU - Ishihara, Yoshihito
AU - Yanagita, T.
AU - Balam, T. A.
AU - Yamashiro, T.
PY - 2012/10
Y1 - 2012/10
N2 - Although human deciduous teeth are an ideal source of adult stem cells, no method for identifying deciduous periodontal ligament (D-PDL) stem cells has so far been developed. In the present study, we investigated whether stage-specific embryonic antigen (SSEA)-4 is a marker that could be used to isolate D-PDL stem cells. The isolated D-PDL cells met the minimum criteria for mesenchymal stem cells (MSCs): They showed plastic adherence, specific-surface antigen expression, and multipotent differentiation potential. SSEA-4+ D-PDL cells were detected in vitro and in vivo. A flow cytometric analysis demonstrated that 22.7% of the D-PDL cells were positive for SSEA-4. SSEA-4+ clonal D-PDL cells displayed multilineage differentiation potential: They were able to differentiate into adipocytes, osteoblasts, and chondrocytes in vitro. A clonal assay demonstrated that 61.5% of the SSEA-4+ D-PDL cells had adipogenic, osteogenic, and chondrogenic potential. Our present study demonstrated that SSEA-4+ D-PDL cells are a subset of multipotent stem cells. Hence, SSEA-4 is a specific marker that can be used to identify D-PDL stem cells.
AB - Although human deciduous teeth are an ideal source of adult stem cells, no method for identifying deciduous periodontal ligament (D-PDL) stem cells has so far been developed. In the present study, we investigated whether stage-specific embryonic antigen (SSEA)-4 is a marker that could be used to isolate D-PDL stem cells. The isolated D-PDL cells met the minimum criteria for mesenchymal stem cells (MSCs): They showed plastic adherence, specific-surface antigen expression, and multipotent differentiation potential. SSEA-4+ D-PDL cells were detected in vitro and in vivo. A flow cytometric analysis demonstrated that 22.7% of the D-PDL cells were positive for SSEA-4. SSEA-4+ clonal D-PDL cells displayed multilineage differentiation potential: They were able to differentiate into adipocytes, osteoblasts, and chondrocytes in vitro. A clonal assay demonstrated that 61.5% of the SSEA-4+ D-PDL cells had adipogenic, osteogenic, and chondrogenic potential. Our present study demonstrated that SSEA-4+ D-PDL cells are a subset of multipotent stem cells. Hence, SSEA-4 is a specific marker that can be used to identify D-PDL stem cells.
KW - adult stem cells
KW - clonal assays
KW - flow cytometry
KW - human deciduous periodontal ligament
KW - multipotential differentiation
KW - stage-specific embryonic antigen-4
UR - http://www.scopus.com/inward/record.url?scp=84866514311&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866514311&partnerID=8YFLogxK
U2 - 10.1177/0022034512458123
DO - 10.1177/0022034512458123
M3 - Article
VL - 91
SP - 955
EP - 960
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
IS - 10
ER -