SSEA-1 marks regionally restricted immature subpopulations of embryonic retinal progenitor cells that are regulated by the Wnt signaling pathway

Hideto Koso, Yasuo Ouchi, Yoko Tabata, Yutaka Aoki, Shinya Satoh, Ken ichi Arai, Sumiko Watanabe

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Identification and expansion of retinal progenitor cells are critical issues from both scientific and clinical aspects. Here, we identified SSEA-1 (CD15) as a novel surface antigen that can be used to define immature retinal progenitor cells. SSEA-1-expressing retinal cells were found in the peripheral region of the early embryonic mouse retina, and then their number dramatically disappeared along with retinal development. FACS analysis showed that the cells strongly positive for SSEA-1 co-expressed Ki67 proliferation antigen in all the developmental stages examined. The SSEA-1-expressing cells formed larger colonies than the non-expressing ones in retinal re-aggregation cultures. Moreover, late onset of rhodopsin expression was observed in SSEA-1-positive progenitor cells, supporting the idea that these cells have an intrinsically immature character. Differential expression of Wnt signal-related genes between SSEA-1-positive and -negative subpopulations of retina cells was revealed, and the expression of constitutively active forms of Wnt signaling molecules resulted in a greater number of SSEA-1-positive cells. In light of all of the data taken together, we propose SSEA-1 to be a surface marker to define a regionally restricted immature subset of progenitor cells of mouse neural retina, with SSEA-1 expression by them positively regulated by Wnt signals.

Original languageEnglish
Pages (from-to)265-276
Number of pages12
JournalDevelopmental Biology
Volume292
Issue number1
DOIs
Publication statusPublished - Apr 1 2006
Externally publishedYes

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CD15 Antigens
Wnt Signaling Pathway
Stem Cells
Retina
Ki-67 Antigen
Rhodopsin
Surface Antigens

Keywords

  • Differentiation
  • FACS
  • Mouse
  • Progenitor cells
  • Proliferation
  • Retina
  • SSEA-1

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

SSEA-1 marks regionally restricted immature subpopulations of embryonic retinal progenitor cells that are regulated by the Wnt signaling pathway. / Koso, Hideto; Ouchi, Yasuo; Tabata, Yoko; Aoki, Yutaka; Satoh, Shinya; Arai, Ken ichi; Watanabe, Sumiko.

In: Developmental Biology, Vol. 292, No. 1, 01.04.2006, p. 265-276.

Research output: Contribution to journalArticle

Koso, Hideto ; Ouchi, Yasuo ; Tabata, Yoko ; Aoki, Yutaka ; Satoh, Shinya ; Arai, Ken ichi ; Watanabe, Sumiko. / SSEA-1 marks regionally restricted immature subpopulations of embryonic retinal progenitor cells that are regulated by the Wnt signaling pathway. In: Developmental Biology. 2006 ; Vol. 292, No. 1. pp. 265-276.
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