Src homology 2 domain of overexpressed Lyn kinase is responsible for the acceleration of granulocyte colony-stimulating factor-induced neutrophilic nuclear lobulation

Naomi Oka, Akiko Suzuki, Tomomi Omura, Hiroshi Sakai, Hiroshi Murakami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have previously shown that the overexpression of a Src family kinase, Lyn, and its kinase-negative form, LynKN, in a granulocyte progenitor cell line, GM-I62M, accelerates neutrophilic nuclear lobulation when the cells are cultured in the presence of granulocyte colony-stimulating factor. In this study, we investigated the role of the Src homology 2 (SH2) and SH3 domains of Lyn in the accelerated induction of nuclear lobulation. In contrast to wild-type Lyn, the overexpression of its SH2 domain mutant did not induce the accelerated nuclear morphological changes, but the overexpressed SH3 domain mutant had the same effects as wild-type Lyn. Therefore, the SH2 domain of Lyn is responsible for the accelerated induction of neutrophilic nuclear lobulation upon G-CSF stimulation.

Original languageEnglish
Pages (from-to)525-532
Number of pages8
JournalCell Biology International
Volume30
Issue number6
DOIs
Publication statusPublished - Jun 1 2006

Keywords

  • Differentiation
  • Granulocyte
  • Lobulation
  • Neutrophil

ASJC Scopus subject areas

  • Cell Biology

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