Spontaneous Structural Changes in Actin Regulate G-F Transformation

Masatoshi Morimatsu, Yuichi Togashi, So Nishikawa, Mitsuhiro Sugawa, Atsuko H. Iwane, Toshio Yanagida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Transformations between G- (monomeric) and F-actin (polymeric) are important in cellular behaviors such as migration, cytokinesis, and morphing. In order to understand these transitions, we combined single-molecule Förster resonance energy transfer with total internal reflection fluorescence microscopy to examine conformational changes of individual actin protomers. We found that the protomers can take different conformational states and that the transition interval is in the range of hundreds of seconds. The distribution of these states was dependent on the environment, suggesting that actin undergoes spontaneous structural changes that accommodate itself to polymerization.

Original languageEnglish
Article numbere45864
JournalPLoS One
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 5 2012
Externally publishedYes

Fingerprint

actin
Actins
Protein Subunits
protein subunits
Cytokinesis
Fluorescence microscopy
Energy Transfer
cytokinesis
energy transfer
fluorescence microscopy
Fluorescence Microscopy
Polymerization
polymerization
Energy transfer
Molecules

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Morimatsu, M., Togashi, Y., Nishikawa, S., Sugawa, M., Iwane, A. H., & Yanagida, T. (2012). Spontaneous Structural Changes in Actin Regulate G-F Transformation. PLoS One, 7(11), [e45864]. https://doi.org/10.1371/journal.pone.0045864

Spontaneous Structural Changes in Actin Regulate G-F Transformation. / Morimatsu, Masatoshi; Togashi, Yuichi; Nishikawa, So; Sugawa, Mitsuhiro; Iwane, Atsuko H.; Yanagida, Toshio.

In: PLoS One, Vol. 7, No. 11, e45864, 05.11.2012.

Research output: Contribution to journalArticle

Morimatsu, M, Togashi, Y, Nishikawa, S, Sugawa, M, Iwane, AH & Yanagida, T 2012, 'Spontaneous Structural Changes in Actin Regulate G-F Transformation', PLoS One, vol. 7, no. 11, e45864. https://doi.org/10.1371/journal.pone.0045864
Morimatsu M, Togashi Y, Nishikawa S, Sugawa M, Iwane AH, Yanagida T. Spontaneous Structural Changes in Actin Regulate G-F Transformation. PLoS One. 2012 Nov 5;7(11). e45864. https://doi.org/10.1371/journal.pone.0045864
Morimatsu, Masatoshi ; Togashi, Yuichi ; Nishikawa, So ; Sugawa, Mitsuhiro ; Iwane, Atsuko H. ; Yanagida, Toshio. / Spontaneous Structural Changes in Actin Regulate G-F Transformation. In: PLoS One. 2012 ; Vol. 7, No. 11.
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